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Insulin–transferrin–selenium (ITS) improves maturation of porcine oocytes in vitro

Published online by Cambridge University Press:  08 February 2011

Junhe Hu
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China. Department of Life Science, Yulin College, Yulin, Shaanxi 719000, China.
Xiaoling Ma
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Jian Chang Bao
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Wei Li
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
De Cheng
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Zhimin Gao
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Anmin Lei
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Chunrong Yang
Affiliation:
College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Huayan Wang*
Affiliation:
Shaanxi Center for Stem Cell Engineering and Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. College of Veterinary Medicine, Shaanxi Key Laboratory for Molecular Biology of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
*
All correspondence to: Huayan Wang, Shaanxi Center for Stem Cell Engineering and Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. Tel: +86 029 87080069. Fax: +86 029 87080068. e-mail: hhwang101@163.com

Summary

The objective of this study was to determine if insulin–transferrin–selenium (ITS) promoted a nuclear and cytoplasmic maturation of porcine oocytes that better supports subsequent embryonic development. The rate of oocyte in vitro maturation (IVM) in an experimental group treated with hormones for 42 h was significantly increased compared with that in a control group without hormone treatment (47.8% vs. 11.7%, respectively, p < 0.05). Following reduction of the hormone treatment period from 42 h to 21 h, which included both the first 21 h period of hormones treatment (45.4%) and the second 21 h period of hormone treatment (44.8%), the rate of oocyte IVM was still higher than that of the control group (p < 0.05). To improve porcine oocyte nuclear maturation, 1% ITS was added to medium supplemented with hormones. The rate of nuclear maturation in the ITS-treated group was significantly higher than in the ITS-untreated group (78.6% vs. 54.4%, respectively, p < 0.05). ITS treatment also significantly reduced the per cent of oocytes with type I and type III cortical granule (CG) distribution, respectively, and significantly increased the per cent of oocytes with type II CG distribution (85.3%). These observations indicated that the synchronization rates of nuclear and ooplasmic maturation reached 67.04% (78.56 × 85.33%). In conclusion, the combination of modified Tissue Culture Medium-199 (mM199) + 10 ng/ml epidermal growth factor (EGF) + 10 IU/ml pregnant mare serum gonadotrophin (PMSG) + 10 IU/ml human chorion gonadotrophin (hCG) + 2.5 IU/ml follicle stimulating hormone (FSH) + 1% ITS is suitable for culturing porcine oocytes in vitro, and effectively enhances porcine oocyte nuclear and cytoplasmic maturation.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2011

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References

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