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Influence of meiotic inhibition by butyrolactone-I during germinal vesicle stage on the ability of porcine oocytes to be activated by electric stimulation after nuclear maturation

Published online by Cambridge University Press:  27 November 2003

Yuji Hirao
Affiliation:
Department of Animal Production and Grasslands Farming, National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020-0198, Japan
Namiko Nishimoto
Affiliation:
Iwate University, Morioka, Iwate 020-0066, Japan
Satoshi Kure-bayashi
Affiliation:
Department of Animal Production and Grasslands Farming, National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020-0198, Japan
Naoki Takenouchi
Affiliation:
Department of Animal Production and Grasslands Farming, National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020-0198, Japan
Nobuhiko Yamauchi
Affiliation:
Department of Developmental Biology, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan
Hiroshi Masuda
Affiliation:
Iwate University, Morioka, Iwate 020-0066, Japan
Takashi Nagai
Affiliation:
Department of Developmental Biology, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan

Abstract

Butyrolactone-I (BL-I) is a specific inhibitor of cyclin-dependent kinases and prevents germinal vesicle breakdown (GVBD) in porcine oocytes. This study first focused on the effect of BL-I on the time course of GVBD and progression to metaphase II (MII) in oocytes after the removal of BL-I. When porcine oocytes were treated with 20 μM BL-I for 28 h, the intervals taken to undergo GVBD and progress to MII were 4-8 h and 16-20 h after washing out BL-I, respectively. These intervals were both approximately 8 h behind those of the control oocytes. When the BL-I treatment was performed for 20 h, instead of 28 h, there were no differences in the timing or frequency of progression to MII between the BL-I treatment and control groups. To determine whether the cytoplasmic maturation was the same, the ability of oocytes to form a female pronucleus in response to an electric stimulus was examined. When oocytes were stimulated at 28 h after the removal of BL-I, the rate of pronucleus formation was significantly lower in oocytes treated with BL-I than in untreated oocytes, despite the fact that both groups reached MII at a similar time. When the electric stimulus was given at 36 h after the removal of BL-I, oocytes had a pronucleus formation rate comparable to that of control oocytes. Therefore, the findings suggest that BL-I treatment of porcine oocytes at the germinal vesicle stage affects not only the progression of meiosis, but also the events involved in cytoplasmic maturation.

Type
Research Article
Copyright
2003 Cambridge University Press

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Influence of meiotic inhibition by butyrolactone-I during germinal vesicle stage on the ability of porcine oocytes to be activated by electric stimulation after nuclear maturation
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Influence of meiotic inhibition by butyrolactone-I during germinal vesicle stage on the ability of porcine oocytes to be activated by electric stimulation after nuclear maturation
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Influence of meiotic inhibition by butyrolactone-I during germinal vesicle stage on the ability of porcine oocytes to be activated by electric stimulation after nuclear maturation
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