Skip to main content Accessibility help
×
Home
Hostname: page-component-78bd46657c-jcldq Total loading time: 0.204 Render date: 2021-05-07T11:29:05.697Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }

Article contents

In vitro development of reconstructed bovine embryos and fate of donor mitochondria following nuclear injection of cumulus cells

Published online by Cambridge University Press:  06 August 2001

Jeong Tae Do
Affiliation:
Animal Resources Research Center, Konkuk University, Kwangjin-gu, Seoul, Korea. Infertility Clinic, Department of Obstetrics and Gynaecology, Kyung Hee University Medical Center, Dongdaemungu, Seoul, Korea.
Kwon Ho Hong
Affiliation:
Animal Resources Research Center, Konkuk University, Kwangjin-gu, Seoul, Korea.
Bo Yon Lee
Affiliation:
Infertility Clinic, Department of Obstetrics and Gynaecology, Kyung Hee University Medical Center, Dongdaemungu, Seoul, Korea.
Seung Bo Kim
Affiliation:
Infertility Clinic, Department of Obstetrics and Gynaecology, Kyung Hee University Medical Center, Dongdaemungu, Seoul, Korea.
Nam-Hyung Kim
Affiliation:
Department of Animal Science, Chungbuk National University, Cheongju, Korea.
Hoon Taek Lee
Affiliation:
Animal Resources Research Center, Konkuk University, Kwangjin-gu, Seoul, Korea.
Kil Saeng Chung
Affiliation:
Animal Resources Research Center, Konkuk University, Kwangjin-gu, Seoul, Korea.

Abstract

In this study we examined the developmental potential of reconstructed embryos and the fate of donor mitochondria during preimplantation development after nuclear transfer in cattle. Isolated cumulus cells were used as donor cells in nuclear transfer. Cumulus cells labelled with MitoTracker Green FM fluorochrome were injected into enucleated bovine MII oocytes and cultured in vitro. MitoTracker labelling on donor cells did not have a detrimental effect on blastocyst formation following nuclear transfer. Cleavage rate was about 69% (56/81) and blastocyst formation rate was 6.2% (5/81) at 7 days after nuclear transfer. The labelled mitochondria dispersed to the cytoplasm and became distributed between blastomeres and could be identified up to the 8- to 15-cell stage. Small patches of mitochondria were detected in some 8- to 15-cell stage embryos (5/20). However, donor mitochondria were not detected in embryos at the 16-cell stage and subsequent developmental stages. In the control group, mitochondria could be identified in arrested 1-cell embryos up to 7 days after nuclear transfer. These results suggest that disappearance of the labelled donor mitochondria in nuclear transfer bovine embryos is not due to fading of the fluorochrome marker, but is rather an as yet undefined cytoplasmic event.

Type
Research Article
Copyright
© 2001 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

In vitro development of reconstructed bovine embryos and fate of donor mitochondria following nuclear injection of cumulus cells
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

In vitro development of reconstructed bovine embryos and fate of donor mitochondria following nuclear injection of cumulus cells
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

In vitro development of reconstructed bovine embryos and fate of donor mitochondria following nuclear injection of cumulus cells
Available formats
×
×

Reply to: Submit a response


Your details


Conflicting interests

Do you have any conflicting interests? *