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GPR120 agonist ameliorated insulin resistance and improved ovarian function

Published online by Cambridge University Press:  09 December 2021

Yang Liu
Affiliation:
Department of Assisted Reproduction, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, 226000, China
Jiayi Ding
Affiliation:
Department of Assisted Reproduction, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, 226000, China
Xiaofang Tan
Affiliation:
Department of Assisted Reproduction, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, 226000, China
Ya Shen
Affiliation:
Department of Assisted Reproduction, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, 226000, China
Li Xu
Affiliation:
Department of Assisted Reproduction, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, 226000, China
Tingting Li
Affiliation:
Department of Assisted Reproduction, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, 226000, China
Weiwei Ma
Affiliation:
Department of Assisted Reproduction, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, 226000, China
Jinting Wu*
Affiliation:
Department of Assisted Reproduction, Affiliated Maternity and Child Health Care Hospital of Nantong University, Jiangsu, 226000, China
*
Author for correspondence: Jinting Wu. 399 Shiji Dadao, Nantong, Jiangsu, 226000, China. Email: wujinting05@163.com

Summary

GPR120 is implicated in the regulation of glucose and lipid metabolism, and insulin resistance. In the current study, we aimed to investigate the role of GPR120 in polycystic ovary syndrome (PCOS). With the adoption of dehydroepiandrosterone, a rat model was established to simulate PCOS in vitro. mRNA and protein expression levels of GPR120 were measured using RT-qPCR and western blot, respectively. In addition, expression levels of testosterone, estradiol, luteinizing hormone and follicle-stimulating hormone, serum total cholesterol and triglyceride were assessed using the corresponding kits. Moreover, haematoxylin and eosin staining was used to detect pathological changes in ovary or liver and oil red staining was utilized to evaluate lipid accumulation. In the present study, GPR120 was downregulated in plasma, liver and ovary in the PCOS rat model. In addition, the GPR120 agonist regulated lipid metabolism in the liver and weight in the PCOS rat model. Furthermore, the GPR120 agonist decreased insulin resistance in the PCOS rat model but improved the ovarian function. It is suggested that GPR120 plays a vital role in suppressing insulin resistance, regulating ovary function and decreasing lipid accumulation in the liver, demonstrating that targeting GPR120 could be an effective method for the improvement of PCOS.

Type
Research Article
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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