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Effect of maturation on the expression of aquaporin 3 in mouse oocyte

Published online by Cambridge University Press:  28 May 2010

Jun Woo Jo ,
Byung Chul Jee ,
Chang Suk Suh ,
Seok Hyun Kim ,
Young Min Choi ,
Jung Gu Kim  and
Shin Yong Moon
Jun Woo Jo
Affiliation:
Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463–707, Korea.
Byung Chul Jee
Affiliation:
Seoul National University Bundang Hospital, Seongnam, Gyeonggi 463–707, Korea. Seoul National University College of Medicine, Seoul 110–744, Korea.
Chang Suk Suh*
Affiliation:
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 300 Gumi, Bundang, Seongnam, Gyeonggi, 463–707, Korea. Seoul National University College of Medicine, Seoul 110–744, Korea. Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul 110–744, Korea.
Seok Hyun Kim
Affiliation:
Seoul National University College of Medicine, Seoul 110–744, Korea. Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul 110–744, Korea.
Young Min Choi
Affiliation:
Seoul National University College of Medicine, Seoul 110–744, Korea. Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul 110–744, Korea.
Jung Gu Kim
Affiliation:
Seoul National University College of Medicine, Seoul 110–744, Korea. Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul 110–744, Korea.
Shin Yong Moon
Affiliation:
Seoul National University College of Medicine, Seoul 110–744, Korea. Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul 110–744, Korea.
*
All correspondence to: Chang Suk Suh. Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 300 Gumi, Bundang, Seongnam, Gyeonggi, 463–707, Korea. Tel: +82 31 787 7251. Fax: + 82 31 787 4054. e-mail: suhcs@snu.ac.kr
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Summary

This study aimed to investigate whether aquaporin 3 (Aqp3) mRNAs are expressed in immature oocytes and altered during in vitro maturation process. Five- to 6-week-old female ICR mice were primed by gonadotropin for 24 and 48 h. Immature oocytes obtained 48 h after priming were also matured in vitro for 17 to 18 h. In vivo matured oocytes were obtained after 48 h priming followed by hCG injection. Total RNAs were extracted from 80 to 150 oocytes in each experimental group, and the levels of Aqp3 mRNA were quantified by real-time reverse transcriptase polymerase chain reaction. The experiments were repeated twice using different oocytes. The Aqp3 mRNA was expressed in immature oocytes, as well as in in vitro and in vivo matured oocytes. The expression level was higher in immature oocytes obtained 48 h after priming (17.2 ± 8.6, mean ± SD) than those with no priming (5.7 ± 0.8) or obtained 24 h after priming (2.5 ± 0.8). The expression of Aqp3 mRNA decreased after in vitro maturation (1.2 ± 0.5), which was similar to in vivo matured oocytes (1.0 ± 0.0). Our work demonstrated that Aqp3 mRNA expression increased during the development of immature oocyte but decreased after completion of in vitro maturation. The results indicate that AQP3 is certainly needed for the acquisition of immature oocytes’ full growing potential within antral follicles.

Keywords

Aquaporin 3Immature oocyteIn vitro maturation
Type
Research Article
Information
Zygote , Volume 19 , Issue 1 , February 2011 , pp. 9 - 14
Copyright
Copyright © Cambridge University Press 2010

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