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Cryoprotective effects of low-density lipoproteins, trehalose and soybean lecithin on murine spermatogonial stem cells

Published online by Cambridge University Press:  14 September 2012

Peng Wang
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Ying Li
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Xiao-chen Hu
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Xiao-Li Cai
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Li-Peng Hou
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Yan-Feng Wang
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Jian-Hong Hu*
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Qing-Wang Li*
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Li-Juan Suo
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Zhi-Guo Fan
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
Bo Zhang
Affiliation:
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China.
*
All correspondence to: Jian-Hong Hu or Qing-Wang Li. College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China. Tel: +86 29 87092102. Fax: +86 29 87092164; e-mail: hjh19732008@yahoo.com.cn or liqingwangysu@yahoo.com.cn
All correspondence to: Jian-Hong Hu or Qing-Wang Li. College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, P.R. China. Tel: +86 29 87092102. Fax: +86 29 87092164; e-mail: hjh19732008@yahoo.com.cn or liqingwangysu@yahoo.com.cn

Summary

Spermatogonial stem cells (SSCs) have the ability to self-renew and offer a pathway for genetic engineering of the male germ line. Cryopreservation of SSCs has potential value for the treatment of male infertility, spermatogonial transplantation, and so on. In order to investigate the cryopreservation effects of different cryoprotectants on murine SSCs, 0.2 M of low-density lipoproteins (LDL), trehalose and soybean lecithin were added to the cryoprotective medium, respectively, and the murine SSCs were frozen at −80°C or −196°C. The results indicated that the optimal recovery rates of murine SSCs in the cryoprotective medium supplemented with LDL, trehalose and soybean lecithin were 92.53, 76.35 and 75.48% at −80°C, respectively. Compared with freezing at −196°C, the optimum temperature for improvement of recovery rates of frozen murine SSCs, cryopreservation in three different cryoprotectants at −80°C, were 17.11, 6.68 and 10.44% respectively. The recovery rates of murine SSCs in the cryoprotective medium supplemented with 0.2 M LDL were significantly higher than that of other cryoprotectants (P < 0.05). Moreover, the recovery rates were demonstrated to be greater at −80°C compared with at −196°C (P < 0.05). In conclusion, 0.2 M of LDL could significantly protect murine SSCs at −80°C. In the freezing–thawing process, LDL is responsible for the cryopreservation of murine SSCs because it can form a protective film at the surface of membranes. However, more research is needed to evaluate and understand the precise role of LDL during the freezing–thawing of SSCs.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2012 

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