Hostname: page-component-848d4c4894-2pzkn Total loading time: 0 Render date: 2024-05-24T21:19:27.568Z Has data issue: false hasContentIssue false

Cat fertilization by mouse sperm injection

Published online by Cambridge University Press:  27 July 2011

Yong-Xun Jin
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Xiang-Shun Cui
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Xian-Feng Yu
Affiliation:
Laboratory Animal Center, Jilin University, Changchun, Jilin Province, 130062, China.
Sung-Hyun Lee
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Qing-Ling Wang
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Wei-Wei Gao
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Yong-Nan Xu
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
Shao-Chen Sun
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
IL-Keun Kong
Affiliation:
Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, GyeongNam, South Korea.
Nam-Hyung Kim*
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea.
*
All correspondence to: Nam-Hyung Kim. Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk 361–763, South Korea. Tel: +82 43 261 2546. Fax: +82 43 272 8853. E-mail: nhkim@chungbuk.ac.kr

Summary

Interspecies intracytoplasmic sperm injection has been carried out to understand species-specific differences in oocyte environments and sperm components during fertilization. While sperm aster organization during cat fertilization requires a paternally derived centriole, mouse and hamster fertilization occur within the maternal centrosomal components. To address the questions of where sperm aster assembly occurs and whether complete fertilization is achieved in cat oocytes by interspecies sperm, we studied the fertilization processes of cat oocytes following the injection of cat, mouse, or hamster sperm. Male and female pronuclear formations were not different in the cat oocytes at 6 h following cat, mouse or hamster sperm injection. Microtubule asters were seen in all oocytes following intracytoplasmic injection of cat, mouse or hamster sperm. Immunocytochemical staining with a histone H3-m2K9 antibody revealed that mouse sperm chromatin is incorporated normally with cat egg chromatin, and that the cat eggs fertilized with mouse sperm enter metaphase and become normal 2-cell stage embryos. These results suggest that sperm aster formation is maternally dependent, and that fertilization processes and cleavage occur in a non-species specific manner in cat oocytes.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Beaujean, N., Taylor, J.E., McGarry, M., Gardner, J.O., Wilmut, I., Loi, P., Ptak, G., Galli, C., Lazzari, G., Bird, A., Young, L.E. & Meehan, R.R. (2004). The effect of interspecific oocytes on demethylation of sperm DNA. Proc. Natl. Acad. Sci. USA 101, 7636–40.CrossRefGoogle ScholarPubMed
Comizzoli, P., Wildt, D.E. & Pukazhenthi, B.S. (2006). Poor centrosomal function of cat testicular spermatozoa impairs embryo development in vitro after intracytoplasmic sperm injection. Biol. Reprod. 75, 252–60.CrossRefGoogle ScholarPubMed
Fulka, H., Barnetova, I., Mosko, T. & Fulka, J. (2008). Epigenetic analysis of human spermatozoa after their injection into ovulated mouse oocytes. Hum Reprod. 23, 627–34.CrossRefGoogle ScholarPubMed
Herrick, J.R., Bond, J.B., Magarey, G.M., Bateman, H.L., Krisher, R.L., Dunford, S.A. & Swanson, W.F. (2007). Toward a feline-optimized culture medium: impact of ions, carbohydrates, essential amino acids, vitamins and serum on development and metabolism of in vitro fertilization-derived feline embryos relative to embryos grown in vivo. Biol. Reprod. 76, 858–70.CrossRefGoogle Scholar
Kim, B.K., Cheon, S.H., Lee, Y.J., Choi, S.H., Cui, X.S. & Kim, N.H. (2003). Pronucleus formation, DNA synthesis and metaphase entry in porcine oocytes following intracytoplasmic injection of murine spermatozoa. Zygote 11, 261–70.CrossRefGoogle ScholarPubMed
Kim, N.H., Chung, H.M., Cha, K.Y. & Chung, K.S. (1998). Microtubule and microfilament organization in maturing human oocytes. Hum. Reprod. 13, 2217–22.CrossRefGoogle ScholarPubMed
Kim, N.H., Jun, S.H., Do, J.T., Uhm, S.J., Lee, H.T. & Chung, K.S. (1999). Intracytoplasmic injection of porcine, bovine, mouse, or human spermatozoon into porcine oocytes. Mol. Reprod. Dev. 53, 8491.3.0.CO;2-U>CrossRefGoogle ScholarPubMed
Kim, N.H., Simerly, C., Funahashi, H., Schatten, G. & Day, B.N. (1996). Microtubule organization in porcine oocytes during fertilization and parthenogenesis. Biol. Reprod. 54, 1397–404.CrossRefGoogle ScholarPubMed
Kimura, Y. & Yanagimachi, R. (1995). Intracytoplasmic sperm injection in the mouse. Biol. Reprod. 52, 709–20.CrossRefGoogle ScholarPubMed
Kimura, Y., Yanagimachi, R., Kuretake, S., Bortkiewicz, H., Perry, A.C. & Yanagimachi, H. (1998). Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material. Biol. Reprod. 58, 1407–15.CrossRefGoogle ScholarPubMed
Morozumi, K. & Yanagimachi, R. (2005). Incorporation of the acrosome into the oocyte during intracytoplasmic sperm injection could be potentially hazardous to embryo development. Proc. Natl. Acad. Sci. 102, 14209–14.CrossRefGoogle ScholarPubMed
Murakami, M., Karja, N.W.K., Wongsrikeao, P., Agung, B., Taniguchi, M., Naoi, H. & Otoi, T. (2005). Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol. Reprod. Dom. Anim. 40, 511–5.CrossRefGoogle ScholarPubMed
Nakamura, S., Terada, Y., Horiuchi, T., Emuta, C., Murakami, T., Yaegashi, N. & Okamura, K. (2001). Human sperm aster formation and pronuclear decondensation in bovine eggs following intracytoplasmic sperm injection using a Piezodriven pipette: a novel assay for human sperm centrosomal function. Biol. Reprod. 65, 1359–63.CrossRefGoogle ScholarPubMed
Oswald, J., Engemann, S., Lane, N., Mayer, W., Olek, A., Fundele, R., Dean, W., Reik, W. & Walter, J. (2000). Active demethylation of the paternal genome in the mouse zygote. Curr. Biol. 20, 475–8.CrossRefGoogle Scholar
Saiki, T. & Hamaguchi, Y. (1992). Mitotic apparatus formation and cleavage induction by micromanipulation of the nucleus and centrosome: the centrosome forms a spindle together with only the chromosomes at a short distance. Exp. Cell. Res. 202, 450–7.CrossRefGoogle Scholar
Saiki, T. & Hamaguchi, Y. (1998). Aster-forming abilities of the egg, polar body, and sperm centrosomes in early starfish development. Dev. Biol. 203, 6274.CrossRefGoogle ScholarPubMed
Schatten, G. (1994). The centrosome and its mode of inheritance: the reduction of the centrosome during gametogenesis and its restoration during fertilization. Dev. Biol. 165, 299335.CrossRefGoogle ScholarPubMed
Simerly, C., Wu, G.J., Zoran, S., Ord, T., Rawlins, R. & Jones, J. (1995). The paternal inheritance of the centrosome, the cell's microtubule-organizing center, in humans, and the implications for infertility. Nat. Med. 1, 4752.CrossRefGoogle ScholarPubMed
Steel, R.G.D. & Torrie, J.H. (1980). Principles and Procedures of Statistics. NY: McGraw Hill Book. Co.Google Scholar
Terada, Y., Simerly, C.R., Hewitson, L. & Schatten, G. (2000). Sperm aster formation and pronuclear decondensation during rabbit fertilization and development of a functional assay for human sperm. Biol. Reprod. 62, 557–63.CrossRefGoogle ScholarPubMed
Yamauchi, Y., Yanagimachi, R. & Horiuchi, T. (2002). Full-term development of golden hamster oocytes following intracytoplasmic sperm head injection. Biol. Reprod. 67, 534–9.CrossRefGoogle ScholarPubMed
Yamazaki, T., Yamagata, K. & Baba, T. (2007). Time-lapse and retrospective analysis of DNA methylation in mouse preimplantation embryos by live cell imaging. Dev. Biol. 304, 409–19.CrossRefGoogle ScholarPubMed
Yanagimachi, R. (2005). Intracytoplasmic injection of spermatozoa and spermatogenic cells: its biology and applications in humans and animals. Reprod. Biomed. Online 10, 247–88.CrossRefGoogle Scholar
Yin, X.J., Lee, Y.H., Jin, J.Y., Kim, N.H. & Kong, I.K. (2006). Nuclear and microtubule remodeling and in vitro development of nuclear transferred cat oocytes with skin fibroblasts of the domestic cat (Felis silvestris catus) and leopard cat (Prionailurus bengalensis). Anim. Reprod. Sci. 95, 307–15.CrossRefGoogle ScholarPubMed