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Article contents

Ascidian sperm receptor attached to the vitelline coat during oocyte maturation

Published online by Cambridge University Press:  16 July 2018

Hitoshi Sawada
Affiliation:
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan
Etsuko Tanaka
Affiliation:
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan
Yukichi Abe
Affiliation:
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan
Satoshi Takizawa
Affiliation:
Department of Life Science, Tokyo Institute of Technology, Nagatsuta Yokohama 226-8501, Japan
Youko Takahashi
Affiliation:
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan
Junko Fujino
Affiliation:
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan
Hideyoshi Yokosawa
Affiliation:
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan

Extract

While all ascidians (Urochordata) are hermaphroditic, some, including Halocynthia roretzi (Fuke, 1983) and Ciona intestinalis (Rosati & De Santis, 1978) are strictly self-sterile because of a self–nonself recognition system in the interaction between the sperm and the vitelline coat of the eggs. However, immature oocytes (Fuke & Numakunai, 1996) and acidic-seawater-treated mature oocytes (Morgan, 1939; Kawamura et al., 1991) are self-fertile. It is thought that a putative self–nonself recognition molecule, which is detached or modified by treatment with acidic seawater, may be attached to the vitelline coat during oocyte maturation. Although the existence of a self–nonself recognition system in the fertilisation process is well known, the molecular entity has yet to be conclusively identified. However, there have been several attempts to identify such a molecule in Ciona (Marino et al., 1999). In the present study, we have isolated and analysed a molecule which appears to be responsible for allorecognition in the interaction between sperm and eggs of the ascidian Halocynthia roretzi.

Biologicals. A solitary ascidian Halocynthia roretzi Type C was used in this study. The fertilisation experiment was carried out as described previously (Sawada et al., 1982).

Isolation and N-terminal Sequencing of Hr VC70. Vitelline coats were isolated from immature and mature oocytes of the ascidian by homogenisation and repeated washing with 5× diluted artificial seawater. The isolated vitelline coats were subjected to SDS-PAGE, followed by blotting to a PVDF membrane. The N-terminal amino acid sequence of the 70 kDa main component (HrVC70) was determined by a protein sequencer.

Type
Special Lecture for Citizens
Copyright
Copyright © Cambridge University Press 1999

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References

Fuke, T.M. (1983). Rouxs Arch. Dev. Biol. 192, 347–52.CrossRefGoogle Scholar
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Fuke, T.M. & Numakunai, T. (1999). Mol. Reprod. Dev. 52, 99106.3.0.CO;2-N>CrossRefGoogle Scholar
Kawamura, K., Nomura, T., Kameda, H., Shimamoto, H. & Nakauchi, M. (1991). Dev. Growth Differ. 33, 139–48.CrossRefGoogle Scholar
Marino, R., De Santis, R., Giuliano, P. & Pinto, M. R. (1999). Proc. Natl. Acad. Sci. USA 96, 9633–6.CrossRefGoogle Scholar
Morgan, T.H. (1939). J. Exp. Zool. 95, 3759.CrossRefGoogle Scholar
Rosati, F. & De Santis, R. (1978). Exp. Cell Res. 112, 111–19.CrossRefGoogle Scholar
Sawada, H., Yokosawa, H., Hoshi, M. & Ishii, S. (1982). Gamete Res. 5, 291301.CrossRefGoogle Scholar
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