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The Parasitic Plant Genome Project: New Tools for Understanding the Biology of Orobanche and Striga

Published online by Cambridge University Press:  20 January 2017

James H. Westwood ,
Claude W. dePamphilis ,
Malay Das ,
Mónica Fernández-Aparicio ,
Loren A. Honaas ,
Michael P. Timko ,
Eric K. Wafula ,
Norman J. Wickett  and
John I. Yoder
James H. Westwood*
Affiliation:
Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061
Claude W. dePamphilis
Affiliation:
Department of Biology, Institute of Molecular Evolutionary Genetics, and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802
Malay Das
Affiliation:
Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061
Mónica Fernández-Aparicio
Affiliation:
Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061 Additional address: Institute for Sustainable Agriculture, IAS-CSIC, Dept. of Plant Breeding, Córdoba, 14080, Spain
Loren A. Honaas
Affiliation:
Department of Biology, Institute of Molecular Evolutionary Genetics, and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802
Michael P. Timko
Affiliation:
Department of Biology, University of Virginia, Charlottesville, VA 22904
Eric K. Wafula
Affiliation:
Department of Biology, Institute of Molecular Evolutionary Genetics, and Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802
Norman J. Wickett
Affiliation:
Department of Plant Sciences, University of California, Davis, CA 95616
*
Corresponding author's E-mail: westwood@vt.edu
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Abstract

The Parasitic Plant Genome Project has sequenced transcripts from three parasitic species and a nonparasitic relative in the Orobanchaceae with the goal of understanding genetic changes associated with parasitism. The species studied span the trophic spectrum from free-living nonparasite to obligate holoparasite. Parasitic species used were Triphysaria versicolor, a photosynthetically competent species that opportunistically parasitizes roots of neighboring plants; Striga hermonthica, a hemiparasite that has an obligate need for a host; and Orobanche aegyptiaca, a holoparasite with absolute nutritional dependence on a host. Lindenbergia philippensis represents the closest nonparasite sister group to the parasitic Orobanchaceae and was included for comparative purposes. Tissues for transcriptome sequencing from each plant were gathered to identify expressed genes for key life stages from seed conditioning through anthesis. Two of the species studied, S. hermonthica and O. aegyptiaca, are economically important weeds and the data generated by this project are expected to aid in research and control of these species and their relatives. The sequences generated through this project will provide an abundant resource of molecular markers for understanding population dynamics, as well as provide insight into the biology of parasitism and advance progress toward understanding parasite virulence and host resistance mechanisms. In addition, the sequences provide important information on target sites for herbicide action or other novel control strategies such as trans-specific gene silencing.

Keywords

Egyptian broomrape, Orobanche aegyptiaca (Pers.) (Syn. Phelipanche aegyptiaca) ORAAELindenbergia philippensis (Cham. & Schltdl.) Benth. LINPHyellowbeak owl's-clover, Triphysaria versicolor (Fisch. & C.A. Mey) TRVEVpurple witchweed, Striga hermonthica, (Del.) Benth. STRHEEST sequencinggene expressionhaustoriumparasitic plant evolutionparasitic weedsRNA-seqtranscriptome sequencingweed genomics
Type
Symposium
Information
Weed Science , Volume 60 , Issue 2 , June 2012 , pp. 295 - 306
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Current address: Institute of Biochemical Plant Pathology, Helmholtz Zentrum Munchen, 85764 Neuherberg, Germany.

References

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