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Fate of Safening Agent L-2-Oxothiazolidine-4-Carboxylic Acid in Sorghum (Sorghum bicolor)

Published online by Cambridge University Press:  12 June 2017

James L. Hilton
Affiliation:
Agric. Res. Serv., U.S. Dep. Agric., Beltsville, MD 20705
Parthasarathy Pillai
Affiliation:
Agric. Res. Serv., U.S. Dep. Agric., Beltsville, MD 20705
Helen A. Norman
Affiliation:
Agric. Res. Serv., U.S. Dep. Agric., Beltsville, MD 20705

Abstract

The herbicide safener OTC (L-2-oxothiazolidine-4-carboxylic acid) increased the amount of reduced thiol compounds in sorghum [Sorghum bicolor (L.) Moench. ‘DK 427′] seedlings. When seedlings were grown in liquid culture medium containing 35S-OTC, the compound was metabolized to radiolabeled cysteine and glutathione. The addition of tridiphane [2-(3,5-dichlorophenyl)-2-(2,2,2-trichloroethyl)oxirane] increased conversion of 35S-OTC to cysteine and resulted in the formation of one additional 35S-labeled compound. When 35S-glutathione was injected into germinating seeds it was converted to 35S-cysteine and both thiols were subsequently found in roots and shoots. Seeds injected with 35S-OTC both translocated the compound to developing roots and shoots and metabolized 35S-OTC to cysteine and glutathione. Excised roots and shoots also metabolized 35S-OTC to the thiols. In an in vitro assay the enzyme 5-oxoprolinase converted OTC to cysteine.

Type
Physiology, Chemistry, and Biochemistry
Copyright
Copyright © 1990 by the Weed Science Society of America 

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References

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