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Proline-106 EPSPS Mutation Imparting Glyphosate Resistance in Goosegrass (Eleusine indica) Emerges in South America

Published online by Cambridge University Press:  18 December 2018

Hudson K. Takano ,
Rafael R. Mendes ,
Leonardo B. Scoz ,
Ramiro F. Lopez Ovejero ,
Jamil Constantin ,
Todd A. Gaines Open the ORCID record for Todd A. Gaines [Opens in a new window] ,
Philip Westra ,
Franck E. Dayan Open the ORCID record for Franck E. Dayan [Opens in a new window]  and
Rubem S. Oliveira Jr
Hudson K. Takano*
Affiliation:
Graduate Student, Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA
Rafael R. Mendes
Affiliation:
Graduate Student, Agronomy Department, State University of Maringá, Paraná, Brazil
Leonardo B. Scoz
Affiliation:
Molecular Biology Researcher, Mato Grosso Cotton Institute, Rondonópolis, Mato Grosso, Brazil
Ramiro F. Lopez Ovejero
Affiliation:
Weed Resistance Management Leader, Monsanto Brazil, São Paulo, Brazil
Jamil Constantin
Affiliation:
Professor, Agronomy Department, State University of Maringá, Paraná, Brazil
Todd A. Gaines
Affiliation:
Assistant Professor, Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA
Philip Westra
Affiliation:
Professor, Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA
Franck E. Dayan
Affiliation:
Professor, Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA
Rubem S. Oliveira Jr
Affiliation:
Professor, Agronomy Department, State University of Maringá, Paraná, Brazil
*
Author for correspondence: Hudson K. Takano, Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523. (Email: hudsontakano@gmail.com)
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Abstract

Glyphosate-resistant (GR) goosegrass [Eleusine indica (L.) Gaertn.] was recently identified in Brazil, but its resistance mechanism was unknown. This study elucidated the resistance mechanism in this species and developed a molecular marker for rapid detection of this target-site resistance trait. The resistance factor for the resistant biotype was 4.4-fold compared with the glyphosate-susceptible (GS) in greenhouse dose–response experiments. This was accompanied by a similar (4-fold) difference in the levels of in vitro and in planta shikimate accumulation in these biotypes. However, there was no difference in uptake, translocation, or metabolism of glyphosate between the GS and GR biotypes. Moreover, both biotypes showed similar values for 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) copy number and transcription. Sequencing of a 330-bp fragment of the EPSPS gene identified a single-nucleotide polymorphism that led to a Pro-106-Ser amino acid substitution in the enzyme from the GR biotype. This mutation imparted a 3.8-fold increase in the amount of glyphosate required to inhibit 50% of EPSPS activity, confirming the role of this amino acid substitution in resistance to glyphosate. A quantitative PCR–based genotyping assay was developed for the rapid detection of resistant plants containing this Pro-106-Ser mutation.

Keywords

Prashant Jha, Montana State University5-enolpyruvylshikimate-3-phosphate synthaseabsorption and translocationEPSPSglyphosate metabolismherbicide-resistance detection, shikimate accumulationtarget-site resistance
Type
Research Article
Information
Weed Science , Volume 67 , Issue 1 , January 2019 , pp. 48 - 56
Copyright
© Weed Science Society of America 2018. 

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