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Glutamate receptor subtypes in human retinal horizontal cells

Published online by Cambridge University Press:  03 May 2004

WEN SHEN ,
SARAH G. FINNEGAN  and
MALCOLM M. SLAUGHTER
WEN SHEN
Affiliation:
Departments of Physiology and Biophysics, University at Buffalo, Buffalo
SARAH G. FINNEGAN
Affiliation:
Department of Neurology, University at Buffalo, Buffalo
MALCOLM M. SLAUGHTER
Affiliation:
Departments of Physiology and Biophysics, University at Buffalo, Buffalo Department of Ophthalmology, University at Buffalo, Buffalo
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Abstract

Glutamate receptor currents were examined in horizontal cells from cultured human retina using whole-cell recording procedures. Horizontal cells possess both AMPA and kainate receptors and both produce significant sustained currents. The kainate-induced current did not show significant desensitization and was not enhanced by concanavalin A. The sustained AMPA current was smaller than the kainate current, but the difference was almost entirely due to pronounced desensitization. The horizontal cell AMPA current was enhanced by cyclothiazide but not by PEPA, indicating the presence of the flip receptor variant. GYKI-52466 blocked the AMPA response (IC50 = 5 μM against 100 μM AMPA) but also blocked the kainate response (IC50 = 45 μM against 100 μM kainate). The diversity of glutamate receptors in human horizontal cells suggests that synaptic input to these neurons may be multiplexed through both kainate and AMPA channels.

Keywords

Kainate receptorsAMPA receptorsGlutamate receptor desensitizationRetinaHorizontal cell
Type
Research Article
Information
Visual Neuroscience , Volume 21 , Issue 1 , January 2004 , pp. 89 - 95
Copyright
2004 Cambridge University Press

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