Massive young stars can be used to trace the current chemical composition of the Galactic disk and to define its metallicity gradient. However, evolutionary models of massive rotating stars predict changes of the surface abundances due to rotationally induced mixing. The abundance analysis of elements sensitive to mixing, such as CNO, can be used to test these rotating stellar models. In this study we identify those stars that show one particular signature of mixing, N-enrichment, in our sample of 70 OB stars for which we have conducted an abundance analysis. This sample is used to define radial metallicity gradients in the Milky Way and we investigate the variation of the calculated nitrogen gradient after the exclusion of these, probably mixing-induced, N-rich stars.