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Metabolic heat and CO2 production rates during germination of melon (Cucumis melo L.) seeds measured by microcalorimetry

Published online by Cambridge University Press:  22 February 2007

Menahem Edelstein ,
Kent J. Bradford  and
David W. Burger
Menahem Edelstein
Affiliation:
Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Kent J. Bradford*
Affiliation:
Department of Vegetable Crops, University of California, Davis, CA 95616-8631, USA
David W. Burger
Affiliation:
Department of Environmental Horticulture, One Shields Avenue, University of California, Davis, CA 95616-8631, USA
*
*Correspondence Fax: +1 530 752 6087 Email: kjbradford@ucdavis.edu Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel. No. 142/2000 series. Supported in part by Western Regional Research Project W-168.
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Abstract

Metabolic heat and CO2 production rates were measured by microcalorimetry during germination of two melon (Cucumis melo L.) accessions, Noy Yizre’el (a cold-sensitive cultivar) and Persia 202 (a cold-tolerant breeding line), differing in their ability to germinate at low temperature (14°C). In seeds that were imbibed at either 14 or 25°C, both heat production rates (φ) and CO2 evolution rates (RCO2) WERE HIGHER IN PERSIA 202 COMPARED TO NOY YIZRE’EL. AT 14°C, Φ AND RCO2of intact Noy Yizre’el seeds remained close to zero and germination was inhibited, but metabolic activity increased with time in decoated seeds (testa removed) and most embryos germinated. The presence of the testa had less effect on metabolic activity or germination of Persia 202 seeds at 14°C. The ratio of φ/RCO2(calorespirometric ratio) is an indication of the substrate being utilized for respiration, with lower values (∼455 kJ mol–1) associated with carbohydrate substrates and higher values (∼800 kJ mol–1) associated with lipid substrates. Removal of the testae of Noy Yizre’el seeds increased φ/RCO2 at 14°C, suggesting that improved oxygen supply to the embryo promotes a shift from carbohydrate to lipid respiratory substrates associated with germination. Consistent with this interpretation, when the hilum apertures of the seed coats were sealed with silicone, φ, RCO2 and φ/RCO2of Noy Yizre’el seeds remained low and germination was inhibited at 25°C, while in Persia 202 seeds the same treatment had relatively little effect on φand RCO2, φ/RCO2approached the value expected for lipid respiratory substrates (700 kJ mol–1), and germination occurred. This confirms earlier anatomical work indicating that the testae of Persia 202 seeds were more porous than were those of Noy Yizre’el seeds, contributing to the greater tolerance of Persia 202 seeds to low temperatures. Abscisic acid (ABA) was also inhibitory to melon seed germination; imbibition of seeds at 25°C in 250 μM ABA decreased φ and RCO2, and φ/RCO2 remained lower than in control seeds in both genotypes. This effect of ABA on metabolic activity could be a cause or consequence of inhibition of germination.

Keywords

abscisic acidcalorimetrymetabolic heat raterespirationseed germinationtemperaturemelonCucumis melo L
Type
Research Article
Information
Seed Science Research , Volume 11 , Issue 3 , September 2001 , pp. 265 - 272
Copyright
Copyright © Cambridge University Press 2001

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References

Alvarado, V., Nonogaki, H. and Bradford, K.J. (2000) Expression of endo-β-mannanase and SNF-related protein kinase genes in true potato seeds in relation to dormancy, gibberellin and abscisic acid. pp. 347–364in Viémont, J.-D.; Crabbé, J. (Eds) Dormancy in plants. From whole plant behavior to cellular control. Wallingford, UK, CABI Publishing.CrossRefGoogle Scholar
Amthor, J.S. (1989) Respiration and crop productivity. New York, Springer-Verlag.CrossRefGoogle Scholar
Bex, J.H.M. (1972) Effects of abscisic acid on oxygen uptake and RNA synthesis in germinating lettuce seeds. Acta Botanica Neerlandica 21, 203–210.CrossRefGoogle Scholar
Connor, K.F., Bonner, F.T. and Vozzo, J.A. (1996) Effects of desiccation on temperate recalcitrant seeds: differential scanning calorimetry, gas chromatography, electron microscopy, and moisture studies on Quercus nigra and Quercus alba. Canadian Journal of Forest Research 26, 1813–1821.CrossRefGoogle Scholar
Coumans, M., Côme, D. and Gaspar, T. (1976) Stabilized dormancy in sugarbeet fruits. I. Seed coats as a physiochemical barrier to oxygen. Botanical Gazette 137, 274–278.CrossRefGoogle Scholar
Criddle, R.S. and Hansen, L.D. (1998) Calorimetric methods for analysis of plant metabolism. pp. 711–763in Gallagher, P.K (series Ed.) Handbook of thermal analysis and calorimetry, Kemp, R.B. (vol. Ed.) Vol. 4: Organic and biological materials. Amsterdam, Elsevier.Google Scholar
Criddle, R.S., Breidenbach, R.W., Rank, D.R., Hopkin, M.S. and Hansen, L.D. (1990) Simultaneous calorimetric and respirometric measurements on plant tissues. Thermochimica Acta 172, 213–221.CrossRefGoogle Scholar
Criddle, R.S., Breidenbach, R.W. and Hansen, L.D. (1991) Plant calorimetry: how to quantitatively compare apples and oranges. Thermochimica Acta 193, 67–90.CrossRefGoogle Scholar
Dunlap, J.R. (1988) Effects of temperature and water potential on the germination of muskmelon cultivars. Annals of Applied Biology 112, 187–194.CrossRefGoogle Scholar
Edelstein, M. and Kigel, J. (1990) Seed germination of melon (Cucumis melo) at sub- and supra-optimal temperatures. Scientia Horticulturae 45, 55–63.CrossRefGoogle Scholar
Edelstein, M., Ben Tal, Y., Wodner, M. and Kigel, J. (1995a) Role of endogenous gibberellins in germination of melon (Cucumis melo) seeds. Physiologia Plantarum 95, 113–119.CrossRefGoogle Scholar
Edelstein, M., Corbineau, F., Kigel, J. and Nerson, H. (1995b) Seed coat structure and oxygen availability control low temperature germination of melon (Cucumis melo) seeds. Physiologia Plantarum 93, 451–456.CrossRefGoogle Scholar
Garciarrubio, A., Legaria, J.P. and Covarrubias, A.A. (1997) Abscisic acid inhibits germination of mature Arabidopsis seeds by limiting the availability of energy and nutrients. Planta 203, 182–187.CrossRefGoogle ScholarPubMed
Gibson, S.I. (2000) Plant sugar-response pathways. Part of a complex regulatory web. Plant Physiology 124, 1532–1539.CrossRefGoogle ScholarPubMed
Grappin, P., Bouinot, D., Sotta, B., Miginiac, E. and Jullien, M. (2000) Control of seed dormancy in Nicotiana plumbaginifolia: post-imbibition abscisic acid synthesis imposes dormancy maintenance. Planta 210, 279–285.CrossRefGoogle ScholarPubMed
Hansen, L.D., Hopkin, M.S. and Criddle, R.S. (1997) Plant calorimetry: a window to plant physiology and ecology. Thermochimica Acta 300, 183–197.CrossRefGoogle Scholar
Heit, C.E. (1951) Germination studies with sensitive muskmelon seedstocks. Proceedings of the Association of Official Seed Analysts 51, 176–182.Google Scholar
Jullien, M., Bouinot, D., Ali-Rachedi, S., Sotta, B. and Grappin, P. (2000) Abscisic acid control of seed dormancy expression in Nicotiana plumbaginifolia and Arabidopsis thaliana. pp. 195–210in Viémont, J.-D.; Crabbé, J. (Eds) Dormancy in plants. From whole plant behavior to cellular control. Wallingford, UK, CABI Publishing.CrossRefGoogle Scholar
Karssen, C.M. (1995) Hormonal regulation of seed development, dormancy, and germination studied by genetic control. pp. 333–350in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Kim, H. (1993) Relationships of tomato (Lycopersicon esculentum Mill.) seed respiration rates to germination rates: effects of reduced water potentials and plant hormones. M.S. Thesis, University of California, Davis.Google Scholar
LePage-Degivry, M.T., Barthe, P. and Garello, G. (1990) Involvement of endogenous abscisic acid in onset and release of Helianthus annuus embryo dormancy. Plant Physiology 92, 1164–1168.CrossRefGoogle Scholar
Pesis, E. and Ng, T.J. (1986) The effect of seedcoat removal on germination and respiration of muskmelon (Cucumis melo) seeds. Seed Science and Technology 14, 117–126.Google Scholar
Prat, H. (1952) Microcalorimetric studies on germination of cereals. Canadian Journal of Botany 30, 379–394.CrossRefGoogle Scholar
Pritchard, H.W., Tompsett, P.B., Manger, K. and Smidt, W.J. (1995) The effect of moisture content on the low temperature responses of Araucaria hunsteinii seed and embryos. Annals of Botany 76, 79–88.CrossRefGoogle Scholar
Reisman-Berman, O., Kigel, J. and Rubin, B. (1989) Short soaking in water inhibits germination of Datura ferox L. and D. stramonium L. seeds. Weed Research 29, 357–363.CrossRefGoogle Scholar
Schopfer, P. and Plachy, C. (1984) Control of seed germination by abscisic acid. II. Effect of embryo water uptake in Brassica napus L. Plant Physiology 76, 155–160.CrossRefGoogle ScholarPubMed
Smeekins, S. (2000) Sugar-induced signal transduction in plants. Annual Review of Plant Physiology and Plant Molecular Biology 51, 49–81.CrossRefGoogle Scholar
Steinbach, H.S., Benech-Arnold, R.L. and Sánchez, R.A. (1997) Hormonal regulation of dormancy in developing sorghum seeds. Plant Physiology 113, 149–154.CrossRefGoogle ScholarPubMed
Teotia, M.S. and Ramakrishna, P. (1984) Chemistry and technology of melon seeds. Journal of Food Science and Technology 21, 332–340.Google Scholar
Thornley, J.H.M. and Johnson, I.R. (1990) Plant and crop modelling. Oxford, Clarendon Press.Google Scholar
Vertucci, C.W. and Farrant, J. (1995) Acquisition and loss of desiccation tolerance. pp. 237–272in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Walters, C. (1998) Understanding the mechanisms and kinetics of seed aging. Seed Science Research 8, 223–244.CrossRefGoogle Scholar
Welbaum, G.E., Tissaoui, T. and Bradford, K.J. (1990) Water relations of seed development and germination in muskmelon (Cucumis melo L.). III. Sensitivity of germination to water potential and abscisic acid during development. Plant Physiology 92, 1029–1037.CrossRefGoogle ScholarPubMed
Yamaguchi, T., Tsukamoto, Y. and Takahashi, K. (1990) Calorimetry as an analytical tool for germination tests of plant seeds. Tokai Journal of Experimental and Clinical Medicine 15, 381–386.Google ScholarPubMed
Yoshioka, T., Endo, T. and Satoh, S. (1998) Restoration of seed germination at supraoptimal temperatures by fluridone, an inhibitor of abscisic acid biosynthesis. Plant and Cell Physiology 39, 307–312.CrossRefGoogle Scholar