Skip to main content Accessibility help
×
Home
Hostname: page-component-99c86f546-t82dr Total loading time: 0.182 Render date: 2021-12-06T03:38:55.803Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }
RNA RNA

Article contents

Mitochondrial minicircles in the free-living bodonid Bodo saltans contain two gRNA gene cassettes and are not found in large networks

Published online by Cambridge University Press:  01 January 2000

DANIËL BLOM
Affiliation:
Department of Biochemistry/AMC, University of Amsterdam, Academic Medical Centre, 1105 AZ, Amsterdam, The Netherlands
ANNETT DE HAAN
Affiliation:
Department of Biochemistry/AMC, University of Amsterdam, Academic Medical Centre, 1105 AZ, Amsterdam, The Netherlands
JANNY VAN DEN BURG
Affiliation:
Department of Biochemistry/AMC, University of Amsterdam, Academic Medical Centre, 1105 AZ, Amsterdam, The Netherlands
MARLENE VAN DEN BERG
Affiliation:
Department of Biochemistry/AMC, University of Amsterdam, Academic Medical Centre, 1105 AZ, Amsterdam, The Netherlands
PAUL SLOOF
Affiliation:
Department of Biochemistry/AMC, University of Amsterdam, Academic Medical Centre, 1105 AZ, Amsterdam, The Netherlands
MILAN JIRKU
Affiliation:
Institute of Parasitology, Czech Academy of Sciences and Faculty of Biology, University of South Bohemia, 37005, Ceské Budejovice, Czech Republic
JULIUS LUKES
Affiliation:
Institute of Parasitology, Czech Academy of Sciences and Faculty of Biology, University of South Bohemia, 37005, Ceské Budejovice, Czech Republic
ROB BENNE
Affiliation:
Department of Biochemistry/AMC, University of Amsterdam, Academic Medical Centre, 1105 AZ, Amsterdam, The Netherlands
Get access

Abstract

In trypanosomatids, the majority of the guide (g) RNAs that provide the information for U-insertion/deletion RNA editing are encoded by minicircles that are catenated into large networks. In contrast, in the distantly related cryptobiid Trypanoplasma borreli, gRNA genes appear to reside in large 180-kb noncatenated DNA circles. To shed light on the evolutionary history and function of the minicircle network, we have analyzed minicircle organization in the free-living bodonid Bodo saltans, which is more closely related to trypanosomatids than T. borreli. We identified 1.4-kb circular DNAs as the B. saltans equivalent of minicircles via sequence analysis of 4 complete minicircles, 14 minicircle fragments, and 14 gRNAs. We show that each minicircle harbors two gRNA gene cassettes of opposite polarity residing in variable regions of about 200 nt in otherwise highly conserved molecules. In the conserved region, B. saltans minicircles contain a putative bent helix sequence and a degenerate dodecamer motif (CSB-3). Electron microscopy, sedimentation, and gel electrophoresis analyses showed no evidence for the existence of large minicircle networks in B. saltans, the large majority of the minicircles being present as circular and linear monomers (85–90%) with small amounts of catenated dimers and trimers. Our results provide the first example of a kinetoplastid species with noncatenated, gRNA gene-containing minicircles, which implies that the creation of minicircles and minicircle networks are separate evolutionary events.

Type
Research Article
Information
RNA , Volume 6 , Issue 1 , January 2000 , pp. 121 - 135
Copyright
© 2000 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Mitochondrial minicircles in the free-living bodonid Bodo saltans contain two gRNA gene cassettes and are not found in large networks
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Mitochondrial minicircles in the free-living bodonid Bodo saltans contain two gRNA gene cassettes and are not found in large networks
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Mitochondrial minicircles in the free-living bodonid Bodo saltans contain two gRNA gene cassettes and are not found in large networks
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *