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Inhibition of viral gene expression by human ribonuclease P

Published online by Cambridge University Press:  01 November 1998

DIANE KAWA
Affiliation:
Program in Infectious Diseases and Immunity, School of Public Health, University of California, Berkeley, California 94720, USA
JUN WANG
Affiliation:
Program in Infectious Diseases and Immunity, School of Public Health, University of California, Berkeley, California 94720, USA
YAN YUAN
Affiliation:
Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
FENYONG LIU
Affiliation:
Program in Infectious Diseases and Immunity, School of Public Health, University of California, Berkeley, California 94720, USA
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Abstract

External guide sequences (EGSs) are small RNA molecules which consist of a sequence complementary to a target mRNA and render the target RNA susceptible to degradation by ribonuclease P (RNase P). EGSs were designed to target the mRNA encoding thymidine kinase (TK) of herpes simplex virus 1 for degradation. These EGSs were shown to be able to direct human RNase P to cleave the TK mRNA sequence efficiently in vitro. A reduction of about 80% in the expression level of both TK mRNA and protein was observed in human cells that steadily expressed an EGS, but not in cells that either did not express the EGS or produced a “disabled” EGS which carried a single nucleotide mutation that precluded RNase P recognition. Thus, EGSs may represent novel gene-targeting agents for inhibition of gene expression and antiviral activity.

Type
Research Article
Information
RNA , Volume 4 , Issue 11 , November 1998 , pp. 1397 - 1406
Copyright
© 1998 RNA Society

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