Skip to main content Accessibility help
×
Home
Hostname: page-component-66d7dfc8f5-hmz2h Total loading time: 0.715 Render date: 2023-02-09T12:34:02.169Z Has data issue: true Feature Flags: { "useRatesEcommerce": false } hasContentIssue true
RNA RNA

Article contents

Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: Implications for binding requirements

Published online by Cambridge University Press:  01 January 1999

ELIN GRAHN
Affiliation:
Department of Molecular Biology, Uppsala University, Box 590, SE-75124 Uppsala, Sweden
NICOLA J. STONEHOUSE
Affiliation:
School of Biology, University of Leeds, Leeds LS29JT, United Kingdom
JAMES B. MURRAY
Affiliation:
School of Biology, University of Leeds, Leeds LS29JT, United Kingdom Present address: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
SJOERD VAN DEN WORM
Affiliation:
Department of Molecular Biology, Uppsala University, Box 590, SE-75124 Uppsala, Sweden Present address: Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, PO Box 9502, NL-2300 RA Leiden, The Netherlands.
KARIN VALEGÅRD
Affiliation:
Department of Molecular Biology, Uppsala University, Box 590, SE-75124 Uppsala, Sweden Present address: Department of Biochemistry, Uppsala University, Box 576, SE-75123 Uppsala, Sweden.
KERSTIN FRIDBORG
Affiliation:
Department of Molecular Biology, Uppsala University, Box 590, SE-75124 Uppsala, Sweden
PETER G. STOCKLEY
Affiliation:
School of Biology, University of Leeds, Leeds LS29JT, United Kingdom
LARS LILJAS
Affiliation:
Department of Molecular Biology, Uppsala University, Box 590, SE-75124 Uppsala, Sweden
Get access

Abstract

The coat protein of bacteriophage MS2 is known to bind specifically to an RNA hairpin formed within the MS2 genome. Structurally this hairpin is built up by an RNA double helix interrupted by one unpaired nucleotide and closed by a four-nucleotide loop. We have performed crystallographic studies of complexes between MS2 coat protein capsids and four RNA hairpin variants in order to evaluate the minimal requirements for tight binding to the coat protein and to obtain more information about the three-dimensional structure of these hairpins. An RNA fragment including the four loop nucleotides and a two-base-pair stem but without the unpaired nucleotide is sufficient for binding to the coat protein shell under the conditions used in this study. In contrast, an RNA fragment containing a stem with the unpaired nucleotide but missing the loop nucleotides does not bind to the protein shell.

Type
Research Article
Information
RNA , Volume 5 , Issue 1 , January 1999 , pp. 131 - 138
Copyright
© 1999 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: Implications for binding requirements
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: Implications for binding requirements
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: Implications for binding requirements
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *