Skip to main content Accessibility help
×
Home
Hostname: page-component-568f69f84b-r4dm2 Total loading time: 0.203 Render date: 2021-09-21T15:57:30.473Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }
RNA RNA

Article contents

The 3′-end-processing factor CPSF is required for the splicing of single-intron pre-mRNAs in vivo

Published online by Cambridge University Press:  29 June 2001

YONGZHONG LI
Affiliation:
Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas 78712, USA Present address: PTC Therapeutics Inc., 100 Corporate Court, South Plainfield, New Jersey 07080, USA.
ZHONG-YING CHEN
Affiliation:
Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas 78712, USA Present address: Johnson & Johnson, Consumer Products Worldwide, 199 Grandview Road, Skillman, New Jersey 08558, USA.
WEIRONG WANG
Affiliation:
Present address: Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey–Robert Wood Johnson Medical School, Piscataway, New Jersey 08855, USA.
CARL C. BAKER
Affiliation:
Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
ROBERT M. KRUG
Affiliation:
Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas 78712, USA
Get access

Abstract

We describe a new approach to elucidate the role of 3′-end processing in pre-mRNA splicing in vivo using the influenza virus NS1A protein. The effector domain of the NS1A protein, which inhibits the function of the CPSF and PABII factors of the cellular 3′-end-processing machinery, is sufficient for the inhibition of not only 3′-end formation but also the splicing of single-intron pre-mRNAs in vivo. We demonstrate that inhibition of the splicing of single-intron pre-mRNAs results from inhibition of 3′-end processing, thereby establishing that 3′-end processing is required for the splicing of a 3′ terminal intron in vivo. Because the NS1A protein causes a global suppression of 3′-end processing in trans, we avoid the ambiguities caused by the activation of cryptic poly(A) sites that occurs when mutations are introduced into the AAUAAA sequence in the pre-mRNA. In addition, this strategy enabled us to establish that the function of a particular 3′-end-processing factor, namely CPSF, is required for the splicing of single-intron pre-mRNAs in vivo: splicing is inhibited only when the effector domain of the NS1A protein binds and inhibits the function of the 30-kDa CPSF protein in 3′-end formation. In contrast, the 3′-end processing factor PABII is not required for splicing. We discuss the implications of these results for cellular and influenza viral mRNA splicing.

Type
Research Article
Copyright
2001 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

The 3′-end-processing factor CPSF is required for the splicing of single-intron pre-mRNAs in vivo
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

The 3′-end-processing factor CPSF is required for the splicing of single-intron pre-mRNAs in vivo
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

The 3′-end-processing factor CPSF is required for the splicing of single-intron pre-mRNAs in vivo
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *