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Rapid Production of Graphite Without Contamination for Biomedical AMS

Published online by Cambridge University Press:  18 July 2016

John S. Vogel
John S. Vogel*
Affiliation:
Center for Accelerator Mass Spectrometry, University of California, Lawrence Livermore National Laboratory, Box 808 L-397, Livermore, California 94551 USA
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Abstract

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The application of AMS to the detection of 14C makes possible a new class of sensitive experiments in molecular biology. Such experiments inherently produce large numbers of samples for the determination of biological variability in molecular interactions. The samples vary in 14C concentration over many orders of magnitude. We added TiH2 to aid the reduction of CO2 by zinc in a sealed tube to reproducibly make graphite without sample cross-contamination. The CO2 is transferred from a combustion tube to the reaction tube through a disposable plastic manifold. The sealed tubes are heated to a single-reaction temperature in a muffle furnace. The process is complete within 5 h. Bulk isotopic fractionation in the finished graphite is less than 0.5%.

Type
I. Sample Preparation and Measurement Techniques
Information
Radiocarbon , Volume 34 , Issue 3: Proceedings of the 14th International Radiocarbon Conference , 1992 , pp. 344 - 350
Copyright
Copyright © The American Journal of Science 

References

Bronk, C. R. and Hedges, R. E. M. 1990 A gaseous ion source for routine AMS radiocarbon dating. In Yiou, F. and Raisbeck, G., eds., Proceedings of the 5th International Conference on Accelerator Mass Spectrometry. Nuclear Instruments and Methods B52: 322–326.CrossRefGoogle Scholar
Boutton, T. W., Wong, W. W., Hachey, D. L., Lee, L. S., Cabrera, M. P. and Klein, P. D. 1983 Comparison of quartz and Pyrex tubes for combustion of organic samples for stable carbon isotope analysis. Analytical Chemistry 55: 1832–1833.Google Scholar
Jull, A. J. T., Donahue, D. J., Hathaway, A. L., Linick, T. W. and Toolin, L. J. 1986 Production of graphite targets by deposition from CO/H2 for precision accelerator 14C measurements. In Stuiver, M. and Kra, R. S., eds., Proceedings of the 12th International 14C Conference. Radiocarbon 28(2A): 191–197.CrossRefGoogle Scholar
Lee, B., Burlitch, J. M. and Hoard, J. L. 1967 The crystal and molecular structure of Zn[Co(CO)4]2 . Journal of the American Chemical Society 89(24): 6362–6363.CrossRefGoogle Scholar
Slota, P. J., Jull, A. J. T., Linick, T. W. and Toolin, L. J. 1987 Preparation of small samples for 14C accelerator targets by catalytic reduction of CO. Radiocarbon 29(2): 303–306.CrossRefGoogle Scholar
Slota, P. J. and Taylor, R. E. 1989 AMS 14C analysis of samples from archaeological contexts: Pretreatment and target preparation. In Ericson, J. E. and Taylor, R. E., eds., University of California Accelerator Mass Spectrometry I, Proceedings of the First U.C. Conference on AMS. Institute of Geophysical and Planetary Physics, University of California, Lawrence Livermore National Laboratory. CONF-8602126: 30–43.Google Scholar
Turteltaub, K. W., Felton, J. S., Gledhill, B. L., Vogel, J. S., Southon, J. R., Caffee, M. W., Finkel, R. C., Nelson, D. E., Proctor, I. P. and Davis, J. C. 1990 Accelerator mass spectrometry in biomedical dosimetry: relationship between low-level exposure and covalent binding of heterocyclic amine-carcinogens to DNA. Proceedings of the National Academy of Sciences 87: 5288–5292.Google Scholar
Turteltaub, K. W., Knize, M. G., Healy, S. K., Tucker, J. D. and Felton, J. S. 1989 The metabolic disposition of 2-amino-1-methyl-6-phenylimidazo (4,5-b) pyridine (PhIP) in the mouse. Journal of Food and Chemical Toxicology 27: 667–673.CrossRefGoogle Scholar
Vogel, J. S., Nelson, D. E. and Southon, J. R. 1989 Accuracy and precision in dating microgram carbon samples. Radiocarbon 31(2): 145–149.Google Scholar
Vogel, J. S., Southon, J. R. and Nelson, D. E. 1990 Memory effects in an AMS system: Catastrophe and recovery. Radiocarbon 32(1): 81–83.Google Scholar