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14C Measurements Elucidate Isotopic Differences between Nails and Hair in Modern Humans

Published online by Cambridge University Press:  26 July 2016

Hector A Martinez De La Torre*
Affiliation:
Earth System Science, University of California, Irvine, B321 Croul Hall, Irvine, California 92697-3100, USA
Kaelyn M Ormsby
Affiliation:
Earth System Science, University of California, Irvine, B321 Croul Hall, Irvine, California 92697-3100, USA
Benjamin T Fuller
Affiliation:
Earth System Science, University of California, Irvine, B321 Croul Hall, Irvine, California 92697-3100, USA
Guaciara M Santos
Affiliation:
Earth System Science, University of California, Irvine, B321 Croul Hall, Irvine, California 92697-3100, USA
*
Corresponding author. Email address: hamartin@uci.edu.

Abstract

In forensic sciences, radiocarbon found in modern human nails and hair is evaluated to determine the year of death. However, 14C analyses presented herein of fingernails and hair from the same infant demonstrated 14C values of hair that were lower than would be expected (e.g. depleted relative to the fingernails by at least 10‰). These results prompted a series of 14C measurements on infant hair strands, fingernails, and infant shampoo, which suggested the presence of C contamination due to cosmetic products. To further evaluate these discrepancies, several hair strands and fingernail samples from multiple donors were collected, pretreated by several approaches, and measured using isotopic analysis (δ13C, δ15N, and C/N as well as 14C accelerator mass spectrometry). SEM images of the surface of chemically pretreated hair strands were also taken to inspect the performance of the chemical pretreatments applied. Our 14C and stable isotope results show that modern human hair is likely contaminated with fossil-fuel-derived carbon, which is found in most hair care products. Currently, the various chemical pretreatments available in the literature and presented herein show that it is not possible to completely remove foreign carbon contaminates. Thus, the human 14C and δ13C values between keratinous tissues (fingernails and hair) arc not in agreement. From these observations, it becomes apparent that isotopic interpretations using human hair should be used with extreme caution and additional work is needed for its use in forensic and dietary research.

Type
Articles
Copyright
Copyright © 2014 by the Arizona Board of Regents on behalf of the University of Arizona 

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References

Arneborg, J, Heinemeier, J, Lynnerup, N, Nielsen, HL, Rud, N, Sveinbjörnsdóttir, ÁE. 1999. Change of diet of the Greenland Vikings determined from stable carbon isotope analysis and 14C dating of their bones. Radiocarbon 41(2):157–68.Google Scholar
Auerswald, K, Rossmann, A, Schäufele, R, Schwertl, M, Monahan, FJ, Schnyder, H. 2011. Does natural weathering change the stable isotope composition (2H, 13C, 15N, 18O and 34S) of cattle hair? Rapid Communications in Mass Spectrometry 25(24):3741–8.Google Scholar
Bergfeld, W, Mulinari-Brenner, F. 2001. Shedding: how to manage a common cause of hair loss. Cleveland Clinic Journal of Medicine 68(3):256–61.Google Scholar
Beverly, RK, Beaumont, W, Tauz, D, Ormsby, KM, von Reden, KF, Santos, GM, Southon, JR. 2010. The Keck Carbon Cycle AMS Laboratory, University of California Irvine: status report. Radiocarbon 52(2):301–9.CrossRefGoogle Scholar
Chen, B-J, Lee, P-L, Chen, W-Y, Mai, F-D, Ling, Y-C. 2006. Hair dye distribution in human hair by ToF-SIMS. Applied Surface Science 252(19):6786–8.Google Scholar
Clausen, T, Schwan-Jonczyk, A, Lang, G, Schuh, W, Liebscher, KD, Springob, C, Franzke, M, Balzer, W, Imhoff, S, Maresch, G. 2006. Hair preparations. In: Ullmann's Encyclopedia of Industrial Chemistry. Wiley VCH. doi: 10.1002/14356007.a12_571.pub2.Google Scholar
Contrino, J, Hair, G, Kreutzer, DL, Rickles, FR. 1996. In situ detection of tissue factor in vascular endothelial cells: correlation with the malignant phenotype of human breast disease. Nature Medicine 2(2):209–15.Google Scholar
DeNiro, MJ. 1985. Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nature 317(6040):806–9.Google Scholar
Evans, T, Wickett, RR. 2012. Practical Modern Hair Science. Carol Stream: Allured Business Media. 562 p.Google Scholar
Feigenbaum, H. 2009. The Use of Cationizing Reagents in the Preparation of Conditioning Polymers for Hair and Skin Care. Saddle Brook: SKW QUAB Chemicals.Google Scholar
Fuller, BT, Fuller, JL, Sage, NE, Harris, DA, O'Connell, TC, Hedges, RE. 2004. Nitrogen balance and δ15N: why you're not what you eat during pregnancy. Rapid Communications in Mass Spectrometry 18(23):2889–96.Google Scholar
Fuller, BT, Fuller, JL, Sage, NE, Harris, DA, O'Connell, TC, Hedges, RE. 2005. Nitrogen balance and δ15N: why you're not what you eat during nutritional stress. Rapid Communications in Mass Spectrometry 19(18):2497–506.Google Scholar
Hao, J, Li, SK. 2008. Mechanistic study of electroosmotic transport across hydrated nail plates: effects of pH and ionic strength. Journal of Pharmaceutical Sciences 97(12):5186–97.Google Scholar
Hodgins, GW. 2009. Measuring atomic bomb-derived 14C levels in human remains to determine year of birth and/or year of death. NIJ Report, 2009. https://www.ncjrs.gov/pdffilesl/nij/grants/227839.pdf.Google Scholar
Hopps, HC. 1977. The biologic bases for using hair and nail for analyses of trace elements. Science of the Total Environment 7(1):7189.Google Scholar
Hua, Q, Barbetti, M, Rakowski, AZ. 2013. Atmospheric radiocarbon for the period 1950–2010. Radiocarbon 55(4):2059–72.Google Scholar
Hunting, AL. 1983. Encyclopedia of Shampoo Ingredients. Cranford, NJ: Micelle Press. 480 p.Google Scholar
Hunting, AL. 1987. Encyclopedia of Conditioning Rinse Ingredients. Cranford: Micelle Press. 506 p.Google Scholar
Jones, R, Chahal, S. 1997. The use of radiolabelling techniques to measure substantivity to, and penetration into, hair of protein hydrolysates. International Journal of Cosmetic Science 19(5):215–26.Google Scholar
Kuzuhara, A, Hori, T. 2003. New method of dyeing keratin fibers using poly (ethylene imine) and its coloring mechanism. Journal of Applied Polymer Science 90(14):3806–10.Google Scholar
Langbein, L, Rogers, MA, Winter, H, Praetzel, S, Beckhaus, U, Rackwitz, H-R, Schweizer, J. 1999. The catalog of human hair keratins. Journal of Biological Chemistry 274(28):19,87484.Google Scholar
Lehn, C, Mutzel, E, Rossmann, A. 2011. Multi-element stable isotope analysis of H, C, N and S in hair and nails of contemporary human remains. International Journal of Legal Medicine 125(5):695706.Google Scholar
Lynnerup, N, Kjeldsen, H, Zweihoff, R, Heegaard, S, Jacobsen, C, Heinemeier, J. 2010. Ascertaining year of birth/age at death in forensic cases: a review of conventional methods and methods allowing for absolute chronology. Forensic Science International 201(1–3):74–8.Google Scholar
Mainkar, A, Jolly, C. 2001. Formulation of natural shampoos. International Journal of Cosmetic Science 23(1):5962.Google Scholar
Meier-Augenstein, W. 1999. Applied gas chromatography coupled to isotope ratio mass spectrometry. Journal of Chromatography A 842(1):351–71.Google Scholar
Meier-Augenstein, W. 2011. Stable Isotope Forensics: An Introduction to the Forensic Application of Stable Isotope Analysis. New York: John Wiley & Sons. 296 p.Google Scholar
Nardoto, GB, Silva, S, Kendall, C, Ehleringer, JR, Chesson, LA, Ferraz, ES, Moreira, MZ, Ometto, JP, Martinelli, LA. 2006. Geographical patterns of human diet derived from stable-isotope analysis of fingernails. American Journal of Physical Anthropology 131(1):137–46.Google Scholar
O'Connell, TC, Hedges, RE. 1999. Investigations into the effect of diet on modern human hair isotopic values. American Journal of Physical Anthropology 108(4):409–25.Google Scholar
O'Connell, TC, Hedges, REM, Healey, MA, Simpson, AHRW. 2001. Isotopic comparison of hair, nail and bone: modern analyses. Journal of Archaeological Science 28(11):1247–55.Google Scholar
Odland, G, Goldsmith, I. 1991. Physiology, Biochemistry and Molecular Biology of the Skin. Oxford: Oxford University Press. Volume 1. p 3.Google Scholar
Oshimura, E, Abe, H, Oota, R. 2007. Hair and amino acids: the interactions and the effects. Journal of Cosmetic Science 58(4):347–57.Google Scholar
Reimer, PJ, Brown, TA, Reimer, RW. 2004. Discussion: reporting and calibration of post-bomb 14C data. Radiocarbon 46(3):1299–304.Google Scholar
Santos, GM, Ormsby, K. 2013. Behavioral variability in ABA chemical pretreatment close to the 14C age limit. Radiocarbon 55(2):534–44.Google Scholar
Santos, GM, Moore, RB, Southon, JR, Griffin, S, Hinger, E, Zhang, D. 2007. AMS 14C sample preparation at the KCCAMS/UCI Facility: status report and performance of small samples. Radiocarbon 49(2):255–69.Google Scholar
Santos, GM, Ormsby, K, Fuller, B. 2011. Can we blame discrepancies of year-of-birth 14C determination on mom? Preliminary isotope results of fingernails of breastfed mother-infant pairs. In: Programme and Abstracts Handbook of the AMS-12 (The Twelfth International Conference on Accelerator Mass Spectrometry). Wellington, New Zealand, p 176.Google Scholar
Schwarcz, HP, Schoeninger, MJ. 1991. Stable isotope analyses in human nutritional ecology. American Journal of Physical Anthropology 34(S13):283321.Google Scholar
Stenström, K, Unkel, I, Nilsson, CM, Rääf, C, Mattsson, S. 2010. The use of hair as an indicator of occupational 14C contamination. Radiation and Environmental Biophysics 49(1):97107.Google Scholar
Taylor, R, Hare, P, Prior, CA, Kirner, DL, Lijun, W, Burky, RR. 1995. Radiocarbon dating of biochemically characterized hair. Radiocarbon 37(2):319–30.Google Scholar
Tsanaclis, L, Wicks, J. 2008. Differentiation between drug use and environmental contamination when testing for drugs in hair. Forensic Science International 176(1):1922.Google Scholar
van Klinken, GJ, Hedges, REM. 1995. Experiments on collagen-humic interactions: speed of humic uptake, and effects of diverse chemical treatments. Journal of Archaeological Science 22(2):263–70.Google Scholar
Villa, AL, Aragão, MR, dos Santos, EP, Mazotto, AM, Zingali, RB, de Souza, EP, Vermelho, AB. 2013. Feather keratin hydrolysates obtained from microbial keratinases: effect on hair fiber. BMC Biotechnology 13(1):15, doi:10.1186/1472-6750-13-15.Google Scholar
Von Braun, J, Diaz-Bonilla, E. 2008. Globalization of Food and Agriculture and the Poor (No. 52). International Food Policy Research Institute (IFPRI). Oxford: Oxford University Press.Google Scholar
Wennig, R. 2000. Potential problems with the interpretation of hair analysis results. Forensic Science International 107(1):512.Google Scholar
Wild, EM, Arlamovsky, KA, Golser, R, Kutschera, W, Priller, A, Puchegger, S, Rom, W, Steier, P, Vycudilik, W. 2000. 14C dating with the bomb peak: an application to forensic medicine. Nuclear Instruments and Methods in Physics Research B 172(1):944–50.Google Scholar
Wilson, A, Dodson, H, Janaway, R, Pollard, A, Tobin, D. 2010. Evaluating histological methods for assessing hair fibre degradation. Archaeometry 52(3):467–81.Google Scholar
Zaias, N. 1990. The Nail in Health and Disease. Norwalk: Appleton & Lange. 181 p.Google Scholar
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