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Removal of the N-terminal hexapeptide from human β2-microglobulin facilitates protein aggregation and fibril formation

Published online by Cambridge University Press:  01 May 2000

G. ESPOSITO
Affiliation:
Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, P. le Kolbe 4, 33100 Udine, Italy
R. MICHELUTTI
Affiliation:
Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, P. le Kolbe 4, 33100 Udine, Italy
G. VERDONE
Affiliation:
Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, P. le Kolbe 4, 33100 Udine, Italy
P. VIGLINO
Affiliation:
Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, P. le Kolbe 4, 33100 Udine, Italy
H. HERNÁNDEZ
Affiliation:
Oxford Centre for Molecular Sciences, University of Oxford, South Parks Road, Oxford OX1 3QT, United Kingdom
C.V. ROBINSON
Affiliation:
Oxford Centre for Molecular Sciences, University of Oxford, South Parks Road, Oxford OX1 3QT, United Kingdom
A. AMORESANO
Affiliation:
Dipartimento di Chimica Organica e Biologica and Centro Internazionale Servizi Spettrometria di Massa, Università di Napoli Federico II, Via Mezzocannone 16, 80124 Napoli, Italy
F. DAL PIAZ
Affiliation:
Dipartimento di Chimica Organica e Biologica and Centro Internazionale Servizi Spettrometria di Massa, Università di Napoli Federico II, Via Mezzocannone 16, 80124 Napoli, Italy
M. MONTI
Affiliation:
Dipartimento di Chimica Organica e Biologica and Centro Internazionale Servizi Spettrometria di Massa, Università di Napoli Federico II, Via Mezzocannone 16, 80124 Napoli, Italy
P. PUCCI
Affiliation:
Dipartimento di Chimica Organica e Biologica and Centro Internazionale Servizi Spettrometria di Massa, Università di Napoli Federico II, Via Mezzocannone 16, 80124 Napoli, Italy
P. MANGIONE
Affiliation:
Dipartimento di Biochimica, Università di Pavia, via Taramelli 3b, 27100 Pavia, Italy Laboratorio di Biotecnologie IRCCS, Policlinico S. Matteo, 27100 Pavia, Italy
M. STOPPINI
Affiliation:
Dipartimento di Biochimica, Università di Pavia, via Taramelli 3b, 27100 Pavia, Italy
G. MERLINI
Affiliation:
Dipartimento di Biochimica, Università di Pavia, via Taramelli 3b, 27100 Pavia, Italy Laboratorio di Biotecnologie IRCCS, Policlinico S. Matteo, 27100 Pavia, Italy
G. FERRI
Affiliation:
Dipartimento di Biochimica, Università di Pavia, via Taramelli 3b, 27100 Pavia, Italy
V. BELLOTTI
Affiliation:
Dipartimento di Biochimica, Università di Pavia, via Taramelli 3b, 27100 Pavia, Italy Laboratorio di Biotecnologie IRCCS, Policlinico S. Matteo, 27100 Pavia, Italy
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Abstract

The solution structure and stability of N-terminally truncated β2-microglobulin (ΔN6β2-m), the major modification in ex vivo fibrils, have been investigated by a variety of biophysical techniques. The results show that ΔN6β2-m has a free energy of stabilization that is reduced by 2.5 kcal/mol compared to the intact protein. Hydrogen exchange of a mixture of the truncated and full-length proteins at μM concentrations at pH 6.5 monitored by electrospray mass spectrometry reveals that ΔN6β2-m is significantly less protected than its wild-type counterpart. Analysis of ΔN6β2-m by NMR shows that this loss of protection occurs in β strands I, III, and part of II. At mM concentration gel filtration analysis shows that ΔN6β2-m forms a series of oligomers, including trimers and tetramers, and NMR analysis indicates that strand V is involved in intermolecular interactions that stabilize this association. The truncated species of β2-microglobulin was found to have a higher tendency to self-associate than the intact molecule, and unlike wild-type protein, is able to form amyloid fibrils at physiological pH. Limited proteolysis experiments and analysis by mass spectrometry support the conformational modifications identified by NMR and suggest that ΔN6β2-m could be a key intermediate of a proteolytic pathway of β2-microglobulin. Overall, the data suggest that removal of the six residues from the N-terminus of β2-microglobulin has a major effect on the stability of the overall fold. Part of the tertiary structure is preserved substantially by the disulfide bridge between Cys25 and Cys80, but the pairing between β-strands far removed from this constrain is greatly perturbed.

Type
Research Article
Copyright
2000 The Protein Society

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Removal of the N-terminal hexapeptide from human β2-microglobulin facilitates protein aggregation and fibril formation
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Removal of the N-terminal hexapeptide from human β2-microglobulin facilitates protein aggregation and fibril formation
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Removal of the N-terminal hexapeptide from human β2-microglobulin facilitates protein aggregation and fibril formation
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