Skip to main content Accessibility help
×
Home
Hostname: page-component-888d5979f-bkf9v Total loading time: 0.272 Render date: 2021-10-26T15:50:55.755Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Relationship between leptin and insulin levels and homeostatic model assessment (HOMA) index in Spanish pubertal children

Published online by Cambridge University Press:  28 January 2009

Stefanie Schoppen
Affiliation:
Lipid Research Laboratory, Fundación Jiménez Díaz, 28040 Madrid, Spain
Beatriz Cano
Affiliation:
Lipid Research Laboratory, Fundación Jiménez Díaz, 28040 Madrid, Spain
Iria de Oya
Affiliation:
Lipid Research Laboratory, Fundación Jiménez Díaz, 28040 Madrid, Spain
Laura López-Simón
Affiliation:
Lipid Research Laboratory, Fundación Jiménez Díaz, 28040 Madrid, Spain
Pia Riestra
Affiliation:
Lipid Research Laboratory, Fundación Jiménez Díaz, 28040 Madrid, Spain
Manuel de Oya
Affiliation:
Lipid Research Laboratory, Fundación Jiménez Díaz, 28040 Madrid, Spain
Carmen Garcés
Affiliation:
Lipid Research Laboratory, Fundación Jiménez Díaz, 28040 Madrid, Spain
Rights & Permissions[Opens in a new window]

Abstract

Type
Abstract
Copyright
Copyright © The Authors 2009

Leptin plays a central role in the maintenance of energy balance(Reference Banks, Farr and Morley1). Leptin concentrations have been shown to be directly proportional to the amount of body fat. However, it is well known that disruption of leptin signalling in the hypothalamus results in obesity. Hyperleptinaemia and/or leptin resistance may play an important role in insulin resistance by participating in its origin. The association between plasma leptin and insulin among adults has been examined(Reference Lawlor, Smith, Kelly, Sattar and Ebrahim2), but studies in pubertal children are scarce.

The aim of the present study was to determine the relationship between plasma leptin and insulin levels and HOMA index in pubertal Spanish boys and girls of normal weight (NW) and overweight (OW).

The study included 356 healthy schoolchildren (165 male and 191 female) from the Comunidad de Madrid, with a mean age of 13.7 years, ranging from 12–15 years, who participated in the follow up of a voluntary survey, the Four Provinces Study, a large-scale cross-sectional study in Spain examining cardiovascular risk factors in four large metropolitan areas of the country(Reference Garcés and de Oya3). The girls and boys were classified into NW and OW children according to Cole et al.(Reference Cole, Bellizzi, Flegal and Dietz4). Obese children were included in the OW group because of their low number. Fasting (12 h) venous blood samples were obtained by venipuncture in Vacutainer tubes. Once centrifuged, fractions were separated and frozen at −70°C. Plasma leptin was determined by ELISA using a commercially-available kit (EIA-2395; DRG Instruments GmbH, Marburg, Germany). Serum insulin concentrations were measured by radioimmunoassay using a commercial kit (BI-Insulin IRMA; Bio-Rad Laboratories, Hercules, CA, USA). HOMA index was calculated (fasting insulin (μU/ml)×fasting glucose (mmol/l)/22.5).

Leptin levels were significantly higher in girls than in boys in NW and OW groups (P<0.0001). Insulin levels and HOMA index were higher in NW girls than NW boys but levels were not different between OW girls and boys. Leptin levels were highly correlated with anthropometric variables in NW and OW girls, while in boys (NW and OW) correlations were weaker, being significant only with BMI. In these pubertal children plasma leptin levels were associated with insulin levels and HOMA index in girls and boys. The correlations were especially strong in girls.

* P<0.05, **P<0.01.

In conclusion, the results demonstrate a relationship between plasma leptin and insulin levels and HOMA index in pubertal children. Furthermore, the correlation between leptin levels and anthropometric data and insulin levels seems to present a gender dimorphism. Further studies in children should focus on the interaction between the extent of obesity, leptin and insulin levels and insulin resistance.

References

1. Banks, WA, Farr, SA & Morley, JE (2006) Physiol Behav 88, 244248.CrossRefGoogle Scholar
2. Lawlor, DA, Smith, GD, Kelly, A, Sattar, N & Ebrahim, S (2007) Obesity 15, 16941701.CrossRefGoogle Scholar
3. Garcés, C & de Oya, M (2007) Rev Esp Cardiol 60, 517524.CrossRefGoogle Scholar
4. Cole, TJ, Bellizzi, MC, Flegal, KM & Dietz, WH (2000) Br Med J 320, 12401243.CrossRefGoogle Scholar
Figure 0

*

You have Access

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Relationship between leptin and insulin levels and homeostatic model assessment (HOMA) index in Spanish pubertal children
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Relationship between leptin and insulin levels and homeostatic model assessment (HOMA) index in Spanish pubertal children
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Relationship between leptin and insulin levels and homeostatic model assessment (HOMA) index in Spanish pubertal children
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *