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The reproduction of in sacco degradation profiles using an in vitro batch culture technique

Published online by Cambridge University Press:  20 November 2017

F.L. Mould ,
K. Kanelias ,
E. Owen  and
E.A. Butler
F.L. Mould
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 237, Reading, RG6 6AR, U.K.
K. Kanelias
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 237, Reading, RG6 6AR, U.K.
E. Owen
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 237, Reading, RG6 6AR, U.K.
E.A. Butler
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 237, Reading, RG6 6AR, U.K.
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Extract

The in sacco technique (e.g. Ørskov et al., 1980) remains the methodology with which the majority of rumen degradability estimates are made. However ethical, animal welfare and cost issues regarding the use of surgically modified animals plus the relatively slow rate at which samples can be examined (two samples per week using three sheep) have combined to compel researchers into developing alternative techniques. Precise, high capacity in vitro systems now exist. However the feed industry is unwilling to adopt these “new” values, partly due to having spent considerable time and effort creating databases of in sacco values which can be used directly in current feed evaluation systems. This paper describes modifications to an in vitro system that provides values similar to that obtained in sacco.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2002 , 2002 , pp. 162
Copyright
Copyright © The British Society of Animal Science 2002

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References

Mould, F.L. and Nordheim, H. 1998. Dry matter and NDF degradation profiles of roughages obtained using the ANKOM in vitro system. Occasional Publication No. 22, British Society of Animal Science 1998, pp 329–331.Google Scholar
Ørskov, E.R. and MacDonald, I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science 92: 499–503.CrossRefGoogle Scholar