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Production of polyclonal antibody for ciprofloxacin detection in Brazilian livestock

Published online by Cambridge University Press:  20 November 2017

S.P. Gobbo
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
C. Longo
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
I.C.S. Bueno
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
K.M.R. Duarte
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
C.F. Meirelles
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
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Extract

The antibiotic use to increase the animal production can result in residual concentrations in products as meat, eggs and milk, exceeding the acceptable doses for human consumption. In Brazil nowadays, 14 antibiotics are employed as feed additive, however there are about 121 drugs used in Veterinary Medicine that contain antibiotics in their formulation. Due to amplitude and frequency of the antimicrobial resistance, it can be considered an emergent and significant question for the public health and an alert for the necessity of a global control about the use of those antibiotics. The immunoassays allow the diagnosis of these antibiotics with accessible costs and presenting good results. The aim of this work was to produce polyclonal antibodies for ciprofloxacin detection using ELISA.

Type
Poster Presentations
Copyright
Copyright © The British Society of Animal Science 2003

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References

Crowther, J.R. 1995. ELISA: Theory and Practice. Methods in Molecular Biology. 42: 223p.Google ScholarPubMed
Duarte, K.M.R.; Gomes, L.H.; Andrino, F.G.; Leal Jr., G.A.; Silva, F.H.B.; Paschoal, J.A.R.; Giacomelli, M.B. and Tavares, F.C.A. 2002. Identificação do vírus do mosaico do tomateiro (TOMV) Tobamovirus, por meio de anticorpos monoclonais. Scientia Agricola, 59: 107112.Google Scholar
Sutula, C.L.; Gillett, J.M.; Morrissey, S.M. and Ramsdell, D.C. 1986. Interpreting ELISA data and establishing the positive-negative threshold. Plant Disease, 70: n.8, 722726.CrossRefGoogle Scholar