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Effect of duration of hormone supplementation on nuclear maturation of porcine oocytes incubated in vitro in a serum-free environment

Published online by Cambridge University Press:  05 November 2021

R. Long ,
M.E. Staines ,
S.A. Edwards ,
P.R. English  and
T.G. McEvoy
R. Long
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK Department of Agriculture, University of Aberdeen, 581 King Street, Aberdeen AB9 1UD, UK
M.E. Staines
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
S.A. Edwards
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
P.R. English
Affiliation:
Department of Agriculture, University of Aberdeen, 581 King Street, Aberdeen AB9 1UD, UK
T.G. McEvoy
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
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Extract

Of the many procedures employed for in vitro maturation (IVM) of mammalian oocytes, most rely on inclusion of serum in the maturation medium. The presence of serum, however, confounds investigations of the effects of discrete factors (nutrients, hormones, etc.) on oocyte development and could pose an additional problem in terms of exposure of oocytes to pathogens (Bavister, 1995). The present study investigated the extent of nuclear maturation of porcine oocytes in vitro in the absence of serum. Nuclear maturation represents the attainment of meiotic maturity and together with cytoplasmic maturation, which facilitates male pronuclear formation following sperm penetration, is a pre-requisite for normal development following fertilization.

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Proceedings of the British Society of Animal Science , Volume 1997 , 1997 , pp. 78
Copyright
Copyright © British Society of Animal Science 1997

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References

Bavister, B.D. (1995). Culture of preimplantation embryos: facts and artefacts. Human Reprod. Update 1: 91–148.Google Scholar
Funahashi, H. and Day, B. N. (1993). Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro . Journal of Reproduction and Fertility 98: 179–185.CrossRefGoogle ScholarPubMed