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Comparison of methods for the measurement of microbial protein supply in sheep

Published online by Cambridge University Press:  05 November 2021

M.A. Samaniego ,
X.B. Chen ,
F.DeB. Hovell ,
J.C. MacRae  and
R. W. Mayes
M.A. Samaniego
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom
X.B. Chen
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom
F.DeB. Hovell
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom
J.C. MacRae
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom
R. W. Mayes
Affiliation:
Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB9 2QL, United Kingdom
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Extract

The excretion of purine derivatives (PD) in urine has been used as an index for the estimation of microbial nitrogen (N) supply in ruminants. This method is simple and does not require surgical intervention of the animal. However, there have been few attempts to compare the results of this method with other methods for the measurement of microbial N supply. In the present study, two experiments were performed. The main experiment (Experiment 2) compared the estimated duodenal microbial N flow based on urinary PD excretion, with the more direct measurement based on duodenal RNA flow rates obtained from cannulated sheep using n-alkanes as the digesta flow marker. However, a preliminary experiment (Experiment 1) was needed to validate the use of alkane as a digesta marker in comparison with the commonly accepted dual phase marker method of Faichney (1975).

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Proceedings of the British Society of Animal Science , Volume 1997 , 1997 , pp. 125
Copyright
Copyright © British Society of Animal Science 1997

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References

Chen, X.B., Chen, Y.K., Franklin, M.F. Ørskov, E.R. and Shand, W.J. 1992. The effect of feed intake and body weight on purine derivative excretion and microbial protein supply in sheep. Journal of Animal Science 70: 1534–1542.CrossRefGoogle ScholarPubMed
Faichney, G. 1975. In ‘Digestion and Metabolism in the Ruminant’, University of New England Publishing Unit, Armidale, New South Wales, Australia, pp. 277–291.Google Scholar
Zinn, R. and Owens, F. 1986. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Canadian Journal of Animal Science 66: 157–166.CrossRefGoogle Scholar