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Tannin bioassay using semi-automated and manual gas production techniques for Brazilian browses

Published online by Cambridge University Press:  20 November 2017

S. L. S. Cabral Filho
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP) CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
I. C. S. Bueno
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP) CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
E. F. Nozella
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP) CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
A. L. Abdalla*
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP) CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
D. M. S. S. Vitti
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP) CP 96, CEP 13400-970, Piracicaba, São Paulo, Brazil.
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Extract

Native herbaceous browses at Northeast of Brazil have a dry tolerance and have been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that can interfere on their intake and digestibility. Polyethylene glycol (PEG) has been used in gas-based techniques for assessing anti nutritional factors in tanniniferous plants for ruminants. The objective of this work was to compare the tannin bioassay technique using the semi-automated (Reading Pressure Technique - RPT) and manual (syringe) gas production techniques to evaluate the tannin effect upon in vitro rumen fermentation, using PEG as an inhibitor of tannin effects.

Type
Sheep Nutrition
Copyright
Copyright © The British Society of Animal Science 2003

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References

Bueno, I. C. S.; Gobbo, S. P.; Abdalla, A. L. and Cabral Filho, S. L. S. 2000. Effect of solid phase of rumen liquor on the inoculum used for in vitro gas production technique. In: Gas production: fermentation kinetics for feed evaluation and to assess microbial activity. EAAP/BSAS, 2122.Google Scholar
Makkar, H. P. S., 2000 Quantification of tannins in tree foliage. Vienna: FAO; IAEA, 2000. cap.3, p.68: Measurement of total phenolics and tannins using Folin-Ciocalteu method. (Laboratory manual).Google Scholar
Mauricio, R. M.; Mould, F. L.; Dhanoa, M. S., ; Owen, E.; Channa, K. S. and Theodorou, M. K., 1999. A semi-automated in vitro gas production technique for ruminant feedstuff evaluation. Animal Feed Science and Technology 79: 32133 CrossRefGoogle Scholar
Menke, K. H.; Raab, L.; Salewiski, A.; Steingass, H.; Fritz, D. and Schneider, W. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro . Journal of Agricultural Science 93: 217222.CrossRefGoogle Scholar

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