Skip to main content Accessibility help
×
Home
Hostname: page-component-5c569c448b-q9r9l Total loading time: 0.167 Render date: 2022-07-02T14:36:13.647Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "useRatesEcommerce": false, "useNewApi": true } hasContentIssue true

Standardization of the immunoassays (ELISA) to detection of gentamicin in livestock using polyclonal antibody

Published online by Cambridge University Press:  20 November 2017

S.P. Gobbo*
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil
P.A. Bricarello
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil
K.M.R. Duarte
Affiliation:
Laboratory of Yeast Genetics – (ESALQ/USP)
S.M.G. Fedrizzi
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil
F.C.A. Tavares
Affiliation:
Laboratory of Yeast Genetics – (ESALQ/USP)
C. F. Meirelles
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil
Get access

Extract

The intensification of cattle in the world and the use of veterinary substances as growing promoters in bovines, sheep, goats and poultry represent a large amount of the total cost of livestock production. Antimicrobial additives when used in animal production for long periods can accumulate residues in animal tissues, including the risk of interactions with other drugs. Global control and monitoring of the use of antibiotics in animal production are significant questions for public health due to the dissemination of microbial resistance to antibiotics drugs. In this study, the objective was to detect the gentamicin antibiotic by indirect PTA-ELISA method (plate trapped antigen. enzyme linked immunoassay), producing polyclonal antibodies from rabbits.

Type
Poster Presentations
Copyright
Copyright © The British Society of Animal Science 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ara, J.; Gans, Z.; Sweeney, R.; Wolf, B., 1995. Dot-ELISA for the rapid detection of gentamicin in milk. Journal of Clinical Laboratory Analytical, 9: 320324.CrossRefGoogle ScholarPubMed
Crowther, J.R. 1995. Methods in Molecular Biology. ELISA: Theory and Practice. 42. Humana Press, Totowa. 223p.CrossRefGoogle ScholarPubMed
Duarte, K.M.R.; Gomes, L.H.; Andrino, F.G.; Leal Jr., G.A.; Silva, F.H.B.; Paschoal, J.A.R.; Giacomelli, M.B. and Tavares, F.C.A, 2002. Identificação do vírus do mosaico do tomateiro (ToMV) Tobamovirus, por meio de anticorpos monoclonais. Scientia Agricola, 59: 107112.CrossRefGoogle Scholar

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Standardization of the immunoassays (ELISA) to detection of gentamicin in livestock using polyclonal antibody
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Standardization of the immunoassays (ELISA) to detection of gentamicin in livestock using polyclonal antibody
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Standardization of the immunoassays (ELISA) to detection of gentamicin in livestock using polyclonal antibody
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *