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Methanogenesis and microbial variation evaluation by qPCR in in vitro gas production test of Lucerne or Tifton hays incubated with BES

Published online by Cambridge University Press:  23 November 2017

A L Abdalla*
Affiliation:
University of Sao Paulo, Piracicaba, SP, Brazil
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Extract

Ruminal fermentation produces methane (CH4), which represents a loss of feed energy and a significant source of greenhouse gas. Researches have been searching for ways of inhibiting ruminal CH4 yield and promising results have been achieved in in vitro assays (Hu et al., 2005). The in vitro gas production technique can predict the in vivo enteric CH4 production from ruminants. Our research focused on the effect of 2-Bromoethanesulfonic acid (BES) on gas and CH4 production and microbial variation of Lucerne and Tifton-85 hays fermented in vitro.

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Poster presentations
Copyright
Copyright © The American Society of International Law 2016

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References

Hu, W.L., Liu, J.X., Ye, J.A., Wu, Y.M., Guo, Y.Q., 2005. Effect of tea saponin on rumen fermentation in vitro . Animal Feed Science and Technology. 120: 333-339.CrossRefGoogle Scholar
Longo, C.; Bueno, I. C. S.; Nozella, E. F.; Godoy, P. B.; Cabral Filho, S. L. S.; Abdalla, A. L., 2005. The influence of head space and inoculum dilution on in vitro ruminal methane measurement. IN: GGAA 2, Zurich, Working papers. p.409-413. Google Scholar
Tavendale, M.H., Meagher, L.P., Pacheco, D., Walker, N., Attwood, G.T. Sivakumaran, S., 2005. Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Animal Feed Science and Technology 123-124: 403-419.Google Scholar

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Methanogenesis and microbial variation evaluation by qPCR in in vitro gas production test of Lucerne or Tifton hays incubated with BES
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