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Gas volume and microbial growth relationship using in vitro techniques related to feed quality

Published online by Cambridge University Press:  20 November 2017

C. Longo*
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
S. P. Gobbo
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
I.C.S. Bueno
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
S.L.S. Cabral Filho
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
A.L. Abdalla
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
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Extract

The gas measured in an in vitro experiment is an indirect measurement of nutritionally important events. Feeds in the rumen are converted to short-chain fatty acids, to CO2, to CH4 and to microbial mass plus water. The pathways used for microorganisms for carbohydrate degradation will determine the amount of ATP available for microbial cell production and vary with the type of substrate, nature of inoculum and time of observation. The aim of this work was to study the relationship between gas volume and microbial growth regarding to the substrate quality.

Type
Poster Presentations
Copyright
Copyright © The British Society of Animal Science 2004

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References

France, J.; Dhanoa, M.S.; Theodorou, M.K.; Lister, S.J.; Davies, S.J. and Isac, D., 1993. A model to interpret gas accumulation profiles with in vitro degradation of ruminant feeds. Journal of Theoretical Biology, 163:99111.CrossRefGoogle Scholar
Orskov, E.R.; McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of American Science, v.92, p.449453.Google Scholar
Theodorou, M.K.; Williams, B.A.; Dhanoa, M.S.; McAllan, A.B. and France, J., 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 48:18597.CrossRefGoogle Scholar
Cabral Filho, S.L.S.; Gobbo, S.P.; Bueno, I.C.S.; Machado, M.C.; Nozella, E.F.; Abdalla, A.L., 2002. Incorporação in vitro de radiofósforo pelos microrganismos ruminais em diferentes proporções do inóculo. Revista Brasileira de Pesquisa e Desenvolvimento, 4 (3): 15381540.Google Scholar
Bueno, I.C.S.; Machado, M.C.; Cabral Filho, S.L.S.; Gobbo, S.P.; Vitti, D.M.S.S.; Abdalla, A.L., 2002. Rumen microbial growth estimation using in vitro radiophosphorous incorporation technique. Revista Brasileira de Pesquisa e Desenvolvimento, 4 (3): 15341537.Google Scholar

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