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Comparison of sheep rumen liquor and Rusitec fluid as inoculum for determining the in vitro digestibility of hays
Published online by Cambridge University Press: 20 November 2017
Extract
One practical disadvantage of some in vitro methods used to estimate the in vivo digestibility of forages is the need for fistulated donor ruminants to provide the rumen liquor. These are subject to restrictive legislation in many countries and are costly to prepare and maintain. The aim of this study was to investigate whether rumen liquor in the in vitro digestibility technique of Van Soest et al. (1966) could be replaced by microorganisms derived from Rusitec. This technique involves the incubation of samples with buffered rumen fluid for 48 h followed by an extraction with a neutral-detergent solution.
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- Copyright © The British Society of Animal Science 2001