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Flavonoid content in different legume germplasm seeds quantified by HPLC

Published online by Cambridge University Press:  01 April 2008

M. L. Wang ,
A. G. Gillaspie ,
J. B. Morris ,
R. N. Pittman ,
J. Davis  and
G. A. Pederson
M. L. Wang*
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
A. G. Gillaspie
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
J. B. Morris
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
R. N. Pittman
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
J. Davis
Affiliation:
Experimental Statistics, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
G. A. Pederson
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
*
*Corresponding author. E-mail: mingli.wang@ars.usda.gov
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Abstract

Flavonoid content was quantified by high performance liquid chromatography (HPLC) and seed-coat colour was recorded from different legume seeds. Soybean seeds generally contained significantly higher amounts of daidzein (315–354 μg/g), genistein (438–458 μg/g), kaempferol (38–68 μg/g) and total measured flavonoids (892–917 μg/g), while cowpea and peanut seeds contained a significantly higher amount of quercetin (214–280 μg/g and 133–289 μg/g, respectively) than the other legumes tested. Significant variation for flavonoid content existed among and within legume species. Daidzein was significantly correlated with genistein and kaempferol (r = 0.92, P < 0.0001; r = 0.68, P < 0.0001), respectively. Genistein was also significantly correlated with kaempferol (r = 0.84, P < 0.0001). Due to differences in genetic background, no consistent relationship was observed between seed-coat colour and flavonoid content. Variation observed in flavonoid content and seed-coat colour would be useful for legume breeding programmes and consumer use.

Keywords

flavonoid contenthigh performance liquid chromatographylegume germplasmseed-coat colour
Type
Research Article
Information
Plant Genetic Resources , Volume 6 , Issue 1 , April 2008 , pp. 62 - 69
Copyright
Copyright © NIAB 2008

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