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Estimating genetic diversity, mating system and pollen dispersal to inform ex situ conservation of the tree Genipa americana L.

Published online by Cambridge University Press:  01 March 2021

Marília Freitas de Vasconcelos Melo ,
Alexandre Magno Sebbenn ,
Bruno Cesar Rossini Open the ORCID record for Bruno Cesar Rossini [Opens in a new window] ,
Ana Veruska Cruz da Silva Muniz ,
Carlos Jose Rodrigues ,
Celso Luis Marino  and
Mario Luiz Teixeira de Moraes
Marília Freitas de Vasconcelos Melo
Affiliation:
Universidade Federal do Alagoas, Campus de Engenharias e Ciências Agrárias, CEP 57100-000, Rio Largo, AL, Brazil
Alexandre Magno Sebbenn
Affiliation:
Instituto Florestal de São Paulo, C.P. 1322, CEP 01059-970, São Paulo, SP, Brazil
Bruno Cesar Rossini*
Affiliation:
Universidade Estadual Paulista, Instituto de Biotecnologia, UNESP, CEP 18607-440, Botucatu, SP, Brazil
Ana Veruska Cruz da Silva Muniz
Affiliation:
Embrapa Tabuleiros Costeiros, CEP 49025-040, Aracaju, SE, Brazil
Carlos Jose Rodrigues
Affiliation:
Cia Energética de São Paulo, Usina Porto Primavera, CEP 19274-000, Primavera, SP, Brazil
Celso Luis Marino
Affiliation:
Universidade Estadual Paulista, Instituto de Biotecnologia, UNESP, CEP 18607-440, Botucatu, SP, Brazil
Mario Luiz Teixeira de Moraes
Affiliation:
Universidade Estadual Paulista, Faculdade de Engenharia, UNESP, CEP 15385-000, Ilha Solteira, SP, Brazil
*
*Corresponding author. E-mail: bruno.rossini@unesp.br
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Abstract

Using microsatellite loci, we assessed the mating system and genetic diversity of the dioecious tropical tree Genipa americana in a natural population (NP) and a progeny test (PT). For NP, we also estimated the paternity correlation within and among fruits and mean pollen dispersal distance. As expected for dioecious species, all offspring originated from outcrossing (t = 1). Mating among relatives (1 − ts) and paternity correlation (rp) were variable among progenies (1 − ts = 0.03–0.19; rp = 0.04–0.40), but greater in NP than in PT. Fixation index (F) was generally significant and lower in adults than in offspring, indicating selection against inbred individuals. Paternity correlation was higher within (0.40) than among (0.26) fruits, indicating a lower effective number of pollen donors (Nep) within (2.5) than among (3.8) fruits. Due to the higher rp in NP, the effective size within progenies (Ne) was lower (2.69) than PT (3.27). The pollen dispersal pattern was strongly leptokurtic, suggesting long-distance pollen dispersal (mean of 179 m). The results show that both populations can be used for seed collection in environmental reforestation programmes; however, considering that PT is structured in maternal progenies, NP is more suitable for seed collection due to the lower probability of mating among related trees.

Keywords

conservation geneticsgene flowmicrosatellite lociprogeny testtropical trees
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 1 , February 2021 , pp. 9 - 19
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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