Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-30T17:07:26.689Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of polymorphic simple-sequence repeat markers that show linkage to a novel sugarcane brown rust disease resistance gene in resistant and susceptible genetic pools

Published online by Cambridge University Press:  12 November 2020

Jie Li ,
Xiao-Yan Wang ,
Hong-Li Shan ,
Rong-Yue Zhang ,
Chan-Mi Wang ,
Xiao-Yan Cang ,
Wen-Feng Li ,
Jiong Yin ,
Zhi-Ming Luo  and
Ying-Kun Huang Open the ORCID record for Ying-Kun Huang [Opens in a new window]
Jie Li
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Xiao-Yan Wang
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Hong-Li Shan
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Rong-Yue Zhang
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Chan-Mi Wang
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Xiao-Yan Cang
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Wen-Feng Li
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Jiong Yin
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Zhi-Ming Luo
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
Ying-Kun Huang*
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan661699, China
*
*Corresponding author. E-mail: huangyk64@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Sugarcane brown rust, caused by Puccinia melanocephala, is one of the main diseases of sugarcane in China. The identification and discovery of new resistance genes have important theoretical and practical significance for preventing outbreaks of brown rust and ensuring the sustainable production of sugarcane. To screen for polymorphic simple-sequence repeat (SSR) molecular markers for localization of brown rust resistance genes, we used two populations that are suitable for genetic linkage map construction and mapping of new resistance genes to construct resistant and susceptible genetic pools. We then screened 449 pairs of primers to identify polymorphic SSR markers in the parental lines and the resistant/susceptible genetic pools. The results showed that 25 pairs of primers directed amplification of polymorphic DNA fragments between the parents of the cross combination ‘Yuetang 03-393’ × ‘ROC 24’, and 16 pairs of primers amplified polymorphic fragments between the parents of the cross combination ‘Liucheng 03-1137’ × ‘Dezhe 93-88’. Four pairs of primers (SMC236CG, SCESSR0928, SCESSR0636 and SCESSR2551) amplified polymorphic DNA fragments between the parental lines and the resistant/susceptible genetic pools in ‘Yuetang 03-393’ × ‘ROC 24’. The results of this study will establish a solid foundation for the mapping of new brown rust resistance genes, genetic linkage map construction and the development of closely-associated molecular markers in sugarcane.

Keywords

brown rustgenetic poolresistance geneSSR markersugarcane
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 6 , December 2020 , pp. 397 - 403
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of NIAB

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally to this study.

References

Asnaghi, C, Roques, D, Ruffel, S, Kaye, C, Hoarau, JY, Télismart, HT, Girard, JC, Raboin, LM, Risterucci, AM, Grivet, L and D'Hont, A (2004) Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers. Theoretical and Applied Genetics 108: 759–764.CrossRefGoogle ScholarPubMed
Comstock, JC, Shine, JM and Raid, RN (1992) Effect of rust on sugarcane growth and biomass. Plant Disease 76: 175–177.10.1094/PD-76-0175CrossRefGoogle Scholar
Cordeiro, GM, Taylor, GO and Henry, RJ (2000) Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploid species. Plant Science 155: 161–168.CrossRefGoogle Scholar
Cunff, LL, Garsmeur, O, Raboin, LM, Pauquet, J, Telismart, H, Selvi, A, Grivet, L, Philippe, R, Begum, D, Deu, M, Costet, L, Wing, R, Glaszmann, JC and D'Hont, A (2008) Diploid/polyploid syntenic shuttle mapping and haplotype–specific chromosome walking toward a rust resistance gene (Bru1) in highly polyploid sugarcane (2n approximately 12× approximately 115). Genetics 180: 649–660.10.1534/genetics.108.091355CrossRefGoogle Scholar
Daugrois, JH, Grivet, L, Roques, D, Hoarau, JY, Lombard, H, Glaszmann, JC and D'Hont, A (1996) A putative major gene for rust resistance linked with a RFLP marker in sugarcane cultivar ‘R570’. Theoretical and Applied Genetics 92: 1059–1064.CrossRefGoogle Scholar
Hoy, J and Hollier, C (2009) Effect of brown rust on yield of sugarcane in Louisiana. Plant Disease 93: 1171–1174.CrossRefGoogle Scholar
Huang, YK and Li, WF (1998) Epidemic and control strategies of sugarcane rust disease in Yunnan sugarcane field. Plant Protection Technology and Extension 18: 22–23 (in Chinese).Google Scholar
Huang, YK and Li, WF (2018) Color Illustration of Diagnosis and Control for Modern Sugarcane Diseases, Pests, and Weeds. Beijing: China Agriculture Press.CrossRefGoogle Scholar
Li, WF, Cai, Q, Huang, YK, Fang, YH and Ma, L (2005) Identification of sugarcane wild germplasm resources resistant to Puccinia erianthi. Plant Protection 31: 51–53 (in Chinese, with English abstract).Google Scholar
Li, WF, Wang, XY, Huang, YK, Zhang, RY, Shan, HL, Yin, J and Luo, ZM (2015a) Identification of brown rust resistance and molecular detection of Bru1 gene in 31 sugarcane wild core germplasm resources. Acta Agronomica Sinica 41: 806–812 (in Chinese, with English abstract).CrossRefGoogle Scholar
Li, WF, Wang, XY, Huang, YK, Shan, HL, Zhang, RY, Yin, J and Luo, ZM (2015b) Identification of resistance to brown rust and molecular detection of gene in 34 sugarcane cultivated original species. Molecular Plant Breeding 13: 1814–1821 (in Chinese, with English abstract).Google Scholar
Li, WF, Wang, XY, Huang, YK, Zhang, HY, Shan, HL, Yin, J and Luo, ZM (2016a) Molecular detection of Bru1 gene and identification of brown rust resistance in Chinese sugarcane germplasm. Sugar Tech: An International Journal of Sugar Crops & Related Industries 19: 183–190.CrossRefGoogle Scholar
Li, WF, Wang, XY, Huang, YK, Zhang, RY, Shan, HL, Luo, ZM and Yin, J (2016b) Identification of resistance to brown rust and molecular detection of Bru1 gene in 101 main sugarcane breeding parents in China. Acta Agronomica Sinica 42: 1411–1416 (in Chinese, with English abstract).CrossRefGoogle Scholar
Li, Z, Xu, LP, Su, YC, Wu, QB, Cheng, W, Sun, TT and Gao, SW (2018) Analysis of brown rust resistance inheritance based on field phenotypes and detection of Bru1 gene in sugarcane. Acta Agronomica Sinica 4: 306–312 (in Chinese, with English abstract).CrossRefGoogle Scholar
Li, XJ, Lin, XQ, Zi, QY, Li, CJ, Xu, CH, Wu, ZD, Zhu, JR, Liu, HB, Fang, ZC and Liu, XL (2019) Cloning and transient expression analysis of ScMOC1 promoter in sugarcane. Journal of Plant Genetic Resources 20: 709–717 (in Chinese, with English abstract).Google Scholar
Molina, BL, Rosales-Longo, F and Queme, JL (2013) Comparative analysis between phenotype and Bru1 marker for incidence to brown rust in sugarcane. Proceedings of International Society of Sugar Cane Technologists 28: 1–4.Google Scholar
Pan, YB (2006) Highly polymorphic microsatellite DNA markers for sugarcane germplasm evaluation and variety identity testing. Sugar Tech: An International Journal of Sugar Crops & Related Industries 8: 246–256.10.1007/BF02943564CrossRefGoogle Scholar
Parco, AS, Avellaneda, MC, Hale, A, Hoy, JW, Kimbeng, CA, Pontif, MJ, Gravois, KA and Baisakh, N (2014) Frequency and distribution of the brown rust resistance gene Bru1 and implications for the Louisiana sugarcane breeding programme. Plant Breeding 133: 654–659.CrossRefGoogle Scholar
Pinto, L, Oliveira, KM, Ulian, EC, Garcia, AA and de Souza, AP (2004) Survey in the sugarcane expressed sequence tag database (SUCEST) for simple sequence repeats. Genome 47: 795–804.CrossRefGoogle ScholarPubMed
Purdy, LH, Liu, LJ and Dean, JL (1983) Sugarcane rust: a newly important disease. Plant Disease 67: 1292–1296.CrossRefGoogle Scholar
Racedo, J, Perera, MF, Bertani, R, Funes, C, Gonzalez, V, Cuenya, MI, D'Hont, A, Welin, B and Castagnaro, AP (2013) Bru1 gene and potential alternative sources of resistance to sugarcane brown rust disease. Euphytica 191: 429–436.CrossRefGoogle Scholar
Raid, RN and Comstock, JC (2000) Common rust. In: Rott, P, Bailey, RA, Comstock, JC, Croft, BJ and Saumtally, AS (eds) A Guide to Sugarcane Diseases. France: La Librairie du Cirad, Montpellier, CIRAD and ISSCT, pp. 85–89.Google Scholar
Rott, PC, Girard, J and Comstook, JC (2013) Impact of pathogen genetics on breeding for resistance to sugarcane diseases. International Society of Sugar Cane Technologists Proceedings 28: 1–11.Google Scholar
Ruan, XY, Yang, F and Sun, CJ (1983) Occurrence of Puccinia erianthi on sugarcane in Yunnan Province. Acta Mycologica Sinica 2: 260–261 (in Chinese).Google Scholar
Santos, FRC, Pinto, LR, Carlini-Garcia, LA, Gazaffi, R, Mancini, MC, Gonçalves, BS, Medeiros, CNF, Perecin, D, Garcia, AAF, Souza, AP and Zucchi, MI (2015) Marker-trait association and epistasis for brown rust resistance in sugarcane. Euphytica 203: 533–547.CrossRefGoogle Scholar
Wang, JP, Roe, B, Macmi, S, Yu, QY, Murray, JE, Tang, HB, Chen, CX, Najar, F, Wiley, G, Bowers, J, Sluys, MAV, Rokhsar, DS, Hudson, ME, Moose, SP, Paterson, AH and Ming, R (2010) Microcollinearity between autopolyploid sugarcane and diploid sorghum genomes. BMC Genomics 11: 261.CrossRefGoogle ScholarPubMed
Wang, XY, Li, WF, Huang, YK, Shan, HL, Zhang, RY, Li, J, Cang, XY, Luo, ZM and Yin, J (2019) Developing genetically segregating populations for localization of novel sugarcane brown rust resistance genes. Euphytica 215: 1–9.CrossRefGoogle Scholar
Wei, JJ, Deng, ZY, Huang, WH, Pan, XH, Wang, BH and Liu, XJ (2010) Control methods and pathogen biological characteristics of sugarcane rust in Beihai. Journal of Anhui Agricultural Sciences 38: 14997–14999 (in Chinese, with English abstract).Google Scholar
Yang, XP, Islam, MS, Sushma, S, Maya, S, Hanson, EA, Comstock, J and Wang, JP (2018) Identifying quantitative trait loci (QTLs) and developing diagnostic markers linked to orange rust resistance in sugarcane (Saccharum spp.). Frontiers in Plant Science 9: 1–10.Google Scholar
Zhang, RY, Li, WF, Huang, YK, Lu, X, Wang, XY, Shan, HL, Li, J, Cang, XY, Yin, J and Luo, ZM (2019) Genetic analysis of sugarcane brown rust resistance genes in wild sugarcane germplasm Erianthus rockii ‘Yundian 95-19’ and Erianthus rockii ‘Yundian 95-20’. Plant Genetic Resources: Characterization and Utilization 17: 460–463.CrossRefGoogle Scholar
Supplementary material: File

Li et al. supplementary material

Li et al. supplementary material

Download Li et al. supplementary material(File)
File 38.9 KB