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Characterization of microsatellite markers for Narcissus dubius, N. cuatrecasasii, N. assoanus and N. rupicola (Amaryllidaceae)

Published online by Cambridge University Press:  23 November 2018

Daniel Barranco Open the ORCID record for Daniel Barranco [Opens in a new window] ,
Violeta I. Simón-Porcar  and
Juan Arroyo
Daniel Barranco*
Affiliation:
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain
Violeta I. Simón-Porcar
Affiliation:
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain
Juan Arroyo
Affiliation:
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain
*
*Corresponding author. E-mail: dnbarranco@gmail.com
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Abstract

The genus Narcissus L. (Amaryllidaceae) provides a model system to study the evolution and maintenance of sexual polymorphisms. In this study, we characterized microsatellite markers for N. dubius, N. cuatrecasasii, N. assoanus and N. rupicola for studies of genetic diversity and paternity analyses to investigate the stability of stylar dimorphism. We proved 40 new primer pairs from a genomic library of N. papyraceus and 12 microsatellite markers characterized also for N. papyraceus in a previous study (52 primer pairs overall). Twenty markers amplified, but their transferability and variability were different among species. Polymorphism was tested at least on 74 individuals and one population per species. The number of polymorphic loci per species ranged from four to eight. The number of alleles per locus ranged from two to 19 and the observed heterozygosity and gene diversity, from 0.107 to 0.729 and 0.103 to 0.894, respectively. These markers can be used for studies of genetic diversity and paternity analyses among individuals of N. dubius, N. cuatrecasasii, N. assoanus and N. rupicola to study the stability of stylar dimorphism.

Keywords

Narcissus dubiusN. cuatrecasasiiN. assoanusN. rupicolaAmaryllidaceaemicrosatellite
Type
Short Communication
Information
Plant Genetic Resources , Volume 17 , Issue 3 , June 2019 , pp. 285 - 288
Copyright
Copyright © NIAB 2018 

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References

Baker, AM, Thompson, JD and Barrett, SCH (2000) Evolution and maintenance of stigma-height dimorphism in Narcissus. I. Floral variation and style-morph ratios. Heredity 84: 502–513.Google Scholar
Barrett, SCH and Harder, LD (2005) The evolution of polymorphic sexual systems in daffodils (Narcissus). New Phytologist 165: 45–53.Google Scholar
Boutin-Ganache, I, Raposo, M, Raymond, M and Deschepper, CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods. BioTechniques 31: 24–28.Google Scholar
Goudet, J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. Journal of Heredity 86: 485–486.Google Scholar
Hanks, GR (2002) Commercial production of Narcissus bulbs. In: Hanks, GR (ed.) Narcissus and Daffodil. London: Taylor and Francis, pp. 53–130.Google Scholar
Herrera, CM (1995) Floral biology, microclimate, and pollination by ectothermic bees in an early-blooming herb. Ecology 76: 218–228.Google Scholar
Hodgins, KA and Barrett, SCH (2008) Asymmetrical mating patterns and the evolution of biased style-morph ratios in a tristylous daffodil. Genetics Research 90: 3–15.Google Scholar
Jones, KC, Levine, KF and Banks, JD (2002) Characterization of 11 polymorphic tetranucleotide microsatellite for forensic applications in California elk (Cervus elaphus canadensis). Molecular Ecology Notes 2: 425–427.Google Scholar
Kington, S (2008) The International Daffodil Registered and Classified List 2008. London: Royal Horticultural Society.Google Scholar
Lloyd, D and Webb, C (1992) The selection of heterostyly. In: Barrett, SCH (ed.) Evolution and Function of Heterostyly. Berlin: Springer-Verlag, pp. 179–207.Google Scholar
Marques, I, Aguilar, JF, Martins-Louçao, MA, Moharrek, F and Feliner, GN (2017) A three-genome five-gene comprehensive phylogeny of the bulbous genus Narcissus (Amaryllidaceae) challenges current classifications and reveals multiple hybridization events. Taxon 66: 832–854.Google Scholar
Peakall, R and Smouse, PE (2012) GenALEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research- an update. Bioinformatics (Oxford, England) 28: 2537–2539.Google Scholar
Pérez-Barrales, R and Arroyo, J (2010) Pollinator shifts and the loss of style polymorphism in Narcissus papyraceus (Amaryllidaceae). Journal of Evolutionary Biology 23: 1117–1128.Google Scholar
Pérez-Barrales, R, Vargas, P and Arroyo, J (2006) New evidence for the Darwinian hypothesis of heterostyly: breeding systems and pollinators in Narcissus sect. Apodanthi. New Phytologist 171: 553–567.Google Scholar
Santos-Gally, R, Gonzalez-Voyer, A and Arroyo, J (2013) Deconstructing heterostyly: the evolutionary role of incompatibility system, pollinators, and floral architecture. Evolution 67: 2072–2082.Google Scholar
Simón, VI, Picó, FX and Arroyo, J (2010) New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species. American Journal of Botany 97: 10–13.Google Scholar
Simón-Porcar, VI, Meagher, TR and Arroyo, J (2015) Disassortative mating prevails in style-dimorphic Narcissus papyraceus despite low reciprocity and compatibility of morphs. Evolution 69: 2276–2288.Google Scholar
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