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Screening for pathotype-specific resistance to broad bean wilt virus 2 and cucumber mosaic virus in pepper (Capsicum annuum L.)

Published online by Cambridge University Press:  17 September 2021

Kyeong-Jae Heo
Affiliation:
Department of International Agricultural Technology, Seoul National University, Pyeongchang25354, Republic of Korea
Boram Choi
Affiliation:
Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang25354, Republic of Korea
Myung-Hwi Kim
Affiliation:
Department of International Agricultural Technology, Seoul National University, Pyeongchang25354, Republic of Korea Integrated Major in Global Smart Farm, Seoul National University, Seoul08826, Republic of Korea
Min-Jun Kwon
Affiliation:
Department of International Agricultural Technology, Seoul National University, Pyeongchang25354, Republic of Korea
Young-Eun Cho
Affiliation:
Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang25354, Republic of Korea
Sun-Jung Kwon
Affiliation:
Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang25354, Republic of Korea
Jang-Kyun Seo*
Affiliation:
Department of International Agricultural Technology, Seoul National University, Pyeongchang25354, Republic of Korea Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang25354, Republic of Korea Integrated Major in Global Smart Farm, Seoul National University, Seoul08826, Republic of Korea
*
Author for correspondence: Jang-Kyun Seo, E-mail: jangseo@snu.ac.kr

Abstract

Two aphid-transmitted RNA viruses, broad bean wilt virus 2 (BBWV2) and cucumber mosaic virus (CMV), are the most prevalent viruses in Korean pepper fields and cause chronic damage in pepper production. In this study, we employed a screening system for pathotype-specific resistance of pepper germplasm to BBWV2 and CMV by utilizing infectious cDNA clones of different pathotypes of the viruses (two BBWV2 strains and three CMV strains). We first examined pathogenic characteristics of the BBWV2 and CMV strains in various plant species and their phylogenetic positions in the virus population structures. We then screened 34 commercial pepper cultivars and seven accessions for resistance. While 21 pepper cultivars were resistant to CMV Fny strain, only two cultivars were resistant to CMV P1 strain. We also found only one cultivar partially resistant to BBWV2 RP1 strain. However, all tested commercial pepper cultivars were susceptible to the resistance-breaking CMV strain GTN (CMV-GTN) and BBWV2 severe strain PAP1 (BBWV2-PAP1), suggesting that breeding new cultivars resistant to these virus strains is necessary. Fortunately, we identified several pepper accessions that were resistant or partially resistant to CMV-GTN and one symptomless accession despite systemic infection with BBWV2-PAP1. These genetic resources will be useful in pepper breeding programs to deploy resistance to BBWV2 and CMV.

Type
Research Article
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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Footnotes

*

These authors contributed equally to the article.

References

Choi, GS, Kim, JH, Lee, DH, Kim, JS and Ryu, KH (2005) Occurrence and distribution of viruses infecting pepper in Korea. Plant Pathology Journal 21, 258–216.10.5423/PPJ.2005.21.3.258CrossRefGoogle Scholar
Choi, GS, Kwon, SJ, Choi, SK, Cho, IS and Yoon, JY (2015) Characteristics of cucumber mosaic virus-GTN and resistance evaluation of chilli pepper cultivars to two cucumber mosaic virus isolates. Research in Plant Disease 21, 99102.10.5423/RPD.2015.21.2.099CrossRefGoogle Scholar
Choi, S, Lee, JH, Kang, WH, Kim, J, Huy, HN, Park, SW, Son, EH, Kwon, JK and Kang, BC (2018) Identification of cucumber mosaic resistance 2 (cmr2) that confers resistance to a new cucumber mosaic virus isolate P1 (CMV-P1) in pepper (Capsicum spp). Frontiers in Plant Science 9, 1106.10.3389/fpls.2018.01106CrossRefGoogle Scholar
Ferrer, RM, Ferriol, I, Moreno, P, Guerri, J and Rubio, L (2011) Genetic variation and evolutionary analysis of broad bean wilt virus 2. Archives of Virology 156, 14451450.10.1007/s00705-011-0990-3CrossRefGoogle ScholarPubMed
Heo, KJ, Kwon, SJ, Kim, MK, Kwak, HR, Han, SJ, Kwon, MJ, Rao, ALN and Seo, JK (2020) Newly emerged resistance-breaking variants of cucumber mosaic virus represent ongoing host-interactive evolution of an RNA virus. Virus Evolution 6, veaa070.10.1093/ve/veaa070CrossRefGoogle ScholarPubMed
Kang, WH, Hoang, NH, Yang, HB, Kwon, JK, Jo, SH, Seo, JK, Kim, KH, Choi, D and Kang, BC (2010) Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L). Theoretical and Applied Genetics 120, 15871596.10.1007/s00122-010-1278-9CrossRefGoogle Scholar
Kang, WH, Seo, JK, Chung, BN, Kim, KH and Kang, BC (2012) Helicase domain encoded by cucumber mosaic virus RNA1 determines systemic infection of Cmr1 in pepper. PLoS ONE 7, e43136.10.1371/journal.pone.0043136CrossRefGoogle ScholarPubMed
Kenyon, L, Kumar, S, Tsai, WS and Hughes, J (2014) Virus diseases of peppers (Capsicum spp.) and their control. Advances in Virus Research 90, 297354.10.1016/B978-0-12-801246-8.00006-8CrossRefGoogle ScholarPubMed
Kim, MK, Seo, JK, Kwak, HR, Kim, JS, Kim, KH, Cha, BJ and Choi, HS (2014) Molecular genetic analysis of cucumber mosaic virus populations infecting pepper suggests unique patterns of evolution in Korea. Phytopathology 104, 9931000.10.1094/PHYTO-10-13-0275-RCrossRefGoogle ScholarPubMed
Kumar, S, Stecher, G, Li, M, Knyaz, C and Tamura, K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35, 15471549.10.1093/molbev/msy096CrossRefGoogle ScholarPubMed
Kwak, HR, Kim, MK, Nam, M, Kim, JS, Kim, KH, Cha, B and Choi, HS (2013) Genetic compositions of broad bean wilt virus 2 infecting red pepper in Korea. Plant Pathology Journal 29, 274284.10.5423/PPJ.OA.12.2012.0190CrossRefGoogle ScholarPubMed
Kwak, HR, Lee, YJ, Kim, J, Kim, MK, Kim, JS, Choi, HS and Seo, JK (2016) A determinant of disease symptom severity is located in RNA2 of broad bean wilt virus 2. Virus Research 211, 2528.10.1016/j.virusres.2015.09.018CrossRefGoogle ScholarPubMed
Kwon, SJ, Cho, IS, Yoon, JY and Chung, BN (2018) Incidence and occurrence pattern of viruses on peppers growing in fields in Korea. Research in Plant Disease 24, 6674.10.5423/RPD.2018.24.1.66CrossRefGoogle Scholar
Lee, YH, Jung, M, Shin, SH, Lee, JH, Choi, SH, Her, NH, Lee, JH, Ryu, KH, Paek, KY and Harn, CH (2009) Transgenic peppers that are highly tolerant to a new CMV pathotype. Plant Cell Reports 28, 223232.10.1007/s00299-008-0637-3CrossRefGoogle ScholarPubMed
Palukaitis, P and Garcia-Arenal, F (2003) Cucumoviruses. Advances in Virus Research 62, 241323.10.1016/S0065-3527(03)62005-1CrossRefGoogle ScholarPubMed
Seo, JK, Kwon, SJ, Choi, HS and Kim, KH (2009) Evidence for alternate states of cucumber mosaic virus replicase assembly in positive- and negative-strand RNA synthesis. Virology 383, 248260.10.1016/j.virol.2008.10.033CrossRefGoogle ScholarPubMed
Seo, JK, Sohn, SH and Kim, KH (2011) A single amino acid change in HC-Pro of soybean mosaic virus alters symptom expression in a soybean cultivar carrying Rsv1 and Rsv3. Archives of Virology 156, 135141.10.1007/s00705-010-0829-3CrossRefGoogle Scholar
Seo, JK, Kwak, HR, Choi, B, Han, SJ, Kim, MK and Choi, HS (2017) Movement protein of broad bean wilt virus 2 serves as a determinant of symptom severity in pepper. Virus Research 242, 141145.CrossRefGoogle ScholarPubMed
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Screening for pathotype-specific resistance to broad bean wilt virus 2 and cucumber mosaic virus in pepper (Capsicum annuum L.)
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