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Genetic diversity and variation analysis of mutant lines derived from γ-ray and chemical mutagen treatments in blackberry (Rubus fruticosus)

Published online by Cambridge University Press:  16 July 2014

Jaihyunk Ryu
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
Bo-Keun Ha
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University, Gwangju500-757, Republic of Korea
Dong Sub Kim
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
Jin-Baek Kim
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
Sang Hoon Kim
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
Joon-Woo Ahn
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
Il Yun Jeong
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
Han-Jik Jo
Affiliation:
Bioplus (Co., Ltd), Wanju-gun, Jeonbuk565-862, Republic of Korea
Ee-Yup Kim
Affiliation:
Bioplus (Co., Ltd), Wanju-gun, Jeonbuk565-862, Republic of Korea
Si-Yong Kang*
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
*
* Corresponding author. E-mail: sykang@kaeri.re.kr

Abstract

Blackberry is a fruiting berry species with very high nutrient contents. With the recent increasing consumer demand for blackberries, new sources of germplasm and breeding techniques are required to improve blackberry production. This study was carried out to evaluate the genetic diversity (GD) and relationship among 55 blackberry (Rubus fruticosus) mutants derived from γ-ray treatment (52 lines) and N-methyl-N′-nitrosourea (MNU) treatment (three lines) using an inter-simple sequence repeat marker. A total of 18 bands were amplified with an average of 3.6 bands per primer. Among them, eight bands were identified to be polymorphic with a rate of 44.4%. In addition, the GD information content values were highest in the 60 Gy treatment population and the GD values were higher in the γ-ray treatment populations than in the MNU treatment population. According to a cluster analysis, all the mutant lines can be classified into five categories, and the genetic distance was greatest between the 80 Gy-irradiated population and other populations. These results indicate that mutant lines have high GD and can be effectively utilized for improving blackberry breeding.

Type
Research Article
Copyright
Copyright © NIAB 2014 

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