Skip to main content Accessibility help
×
Home
Hostname: page-component-7ccbd9845f-s2vjv Total loading time: 0.35 Render date: 2023-01-31T02:06:56.176Z Has data issue: true Feature Flags: { "useRatesEcommerce": false } hasContentIssue true

Diversity of the W1 gene encoding flavonoid 3′,5′-hydroxylase in white- and purple-flowered soybeans

Published online by Cambridge University Press:  08 December 2014

Gyu Tae Park
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Jagadeesh Sundaramoorthy
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Jong-Beum Park
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Jeong Dong Lee
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Kwang Shik Choi
Affiliation:
Department of Biology, Kyungpook National University, Daegu702-701, Republic of Korea
Jeong Hoe Kim
Affiliation:
Department of Biology, Kyungpook National University, Daegu702-701, Republic of Korea
Hak Soo Seo
Affiliation:
Department of Plant Bioscience, Seoul National University, Seoul151-742, Republic of Korea Bio-MAX Institute, Seoul National University, Seoul151-818, Republic of Korea
Soon-Ki Park
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Jong Tae Song*
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
*
*Corresponding author. E-mail: jtsong68@knu.ac.kr

Abstract

Cultivated soybeans [Glycinemax (L.) Merr.] have various flower colours such as dark purple, purple, light purple, pink, magenta, near white and white. About one-third of the soybean accessions in the United States Department of Agriculture – Germplasm Resource Information Network (USDA-GRIN) Soybean Germplasm Collections have white flowers and are the second dominant accessions after the purple-flowered accessions. Earlier studies have shown that the w1 recessive allele of the W1 gene encoding flavonoid 3′,5′-hydroxylase produces white flowers. In the present study, we aimed to understand why the white-flowered accessions have become abundant among the cultivated soybeans and what their genetic and regional origin is. For this purpose, 99 landraces with white flowers and 39 landraces with purple flowers from eight Asian countries and Russia were analysed with regard to the nucleotide sequences of the W1 locus. We not only found that the w1 alleles of the 99 white-flowered landraces were identical to those of the white-flowered Williams 82, but also found that these w1 alleles displayed no polymorphism at all. By carrying out a phylogenetic analysis, we were able to identify a group with W1 alleles from which the w1 allele might have diverged.

Type
Research Article
Copyright
Copyright © NIAB 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abe, J, Hasegawa, A, Fukushi, H, Mikami, T, Ohara, M and Shimamoto, Y (1999) Introgression between wild and cultivated soybeans of Japan revealed by RFLP analysis for chloroplast DNAs. Economic Botany 53: 285291.CrossRefGoogle Scholar
An, W, Zhao, H, Dong, YS, Wang, Y, Li, Q, Zhuang, BC, Gong, L and Liu, B (2009) Genetic diversity in annual wild soybean (Glycine soja Sieb. et Zucc.) and cultivated soybean (G. max. Merr.) from different latitudes in China. Pakistan Journal of Botany 41: 22292242.Google Scholar
Buzzell, RI, Buttery, BR and MacTavish, DC (1987) Biochemical genetics of black pigmentation of soybean seed. Journal of Heredity 78: 5354.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 1115.Google Scholar
Fasoula, DA, Stephens, PA, Nickell, CD and Vodkin, LO (1995) Cosegregation of purple-throat flower with dihydroflavonol-reductase polymorphism in soybean. Crop Science 35: 10281031.CrossRefGoogle Scholar
Guindon, S, Dufayard, JF, Lefort, V, Anisimova, M, Hordijk, W and Gascuel, O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59: 307321.CrossRefGoogle ScholarPubMed
Han, O, Abe, J and Shimamoto, Y (1999) Genetic diversity of soybean landraces in Korea. Korean Journal of Crop Science 44: 256262.Google Scholar
Hartwig, EE and Hinson, K (1962) Inheritance of flower color of soybeans. Crop Science 2: 152153.CrossRefGoogle Scholar
Hymowitz, T and Kaizuma, N (1981) Soybean seed protein electrophoresis profiles from 15 Asian countries or regions: hypotheses on paths of dissemination of soybeans from China. Economic Botany 35: 1023.CrossRefGoogle Scholar
Johnson, EOC, Stephens, PA, Fasoula, DA, Nickell, CD and Vodkin, LO (1998) Instability of a novel multicolored flower trait in inbred and outcrossed soybean lines. Journal of Heredity 89: 508515.CrossRefGoogle Scholar
Kim, MY, Lee, S, Van, K, Kim, TH, Jeong, SC, Choi, IY, Kim, DS, Lee, YS, Park, D, Ma, J, Kim, WY, Kim, BC, Park, S, Lee, KA, Kim, DH, Kim, KH, Shin, JH, Jang, YE, Kim, KD, Liu, WX, Chaisan, T, Kang, YJ, Lee, YH, Kim, KH, Moon, JK, Schmutz, J, Jackson, SA, Bhak, J and Lee, SH (2010) Whole-genome sequencing and intensive analysis of the undomesticated soybean (Glycine soja Sieb. and Zucc.) genome. Proceedings of the National Academy of Science, USA 107: 2203222037.CrossRefGoogle ScholarPubMed
Kuroda, Y, Kaga, A, Tomooka, N and Vaughan, DA (2006) Population genetic structure of Japanese wild soybean (Glycine soja) based on microsatellite variation. Molecular Ecology 15: 959974.CrossRefGoogle ScholarPubMed
Lam, HM, Xu, X, Liu, X, Chen, W, Yang, G, Wong, FL, Li, MW, He, W, Qin, N, Wang, B, Li, J, Jian, M, Wang, J, Shao, G, Wang, J, Sun, SS and Zhang, G (2010) Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection. Nature Genetics 42: 10531059.CrossRefGoogle Scholar
Li, YH, Li, W, Zhang, C, Yang, L, Chang, RZ, Gaut, BS and Qiu, LJ (2010) Genetic diversity in domesticated soybean (Glycine max) and its wild progenitor (Glycine soja) for simple sequence repeat and single-nucleotide polymorphism loci. New Phytologist 188: 242253.CrossRefGoogle ScholarPubMed
Maughan, PJ, Saghai-Maroof, MA, Buss, GR and Huestis, GM (1996) Amplified fragment length polymorphism (AFLP) in soybean: species diversity, inheritance, near-isogenic line analysis. Theoretical and Applied Genetics 93: 392401.CrossRefGoogle ScholarPubMed
Ortiz-Perez, E, Horner, HT, Hanlin, SJ and Palmer, RG (2006) Evaluation of Insect-mediated seed set among soybean lines segregating for male sterility at the ms6 locus. Field Crops Research 97: 353362.CrossRefGoogle Scholar
Palmer, RG and Groose, RW (1993) A new allele at the w4 locus derived from the w4-m mutable allele in soybean. Journal of Heredity 84: 297300.CrossRefGoogle Scholar
Palmer, RG, Pfeiffer, TW, Buss, GR and Kilen, TC (2004) Qualitative genetics. In: Specht, JE and Boerma, HR (eds) Soybean: Improvement, Production, and Uses. Agronomy Monograph 16 . 3rd edn. Madison, Wisconsin: American Society of Agronomy Inc, pp. 137234.Google Scholar
Posada, D (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25: 12531256.CrossRefGoogle ScholarPubMed
Powell, W, Morgante, M, Doyle, JJ, McNicol, JW, Tingey, SV and Rafalski, AJ (1996) Genepool variation in genus Glycine subgenus soja revealed by polymorphic nuclear and chloroplast microsatellites. Genetics 144: 793803.Google ScholarPubMed
Rozas, J, Sanchez-DelBarrio, JC, Messeguer, X and Rozas, R (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19: 24962497.CrossRefGoogle ScholarPubMed
Severson, DW (1983) Honey bees and soybeans: analyses of floral chemistry relating to foraging preferences. PhD Thesis, University of Wisconsin-Madison.Google Scholar
Stephens, PA and Nickell, CD (1992) Inheritance of pink flower in soybean. Crop Science 32: 11311132.CrossRefGoogle Scholar
Takahashi, R, Githiri, SM, Hatayama, K, Dubouzet, EG, Shimada, N, Aoki, T, Ayabe, S, Iwashina, T, Toda, K and Matsumura, H (2007) A single-base deletion in soybean flavonol synthase gene is associated with magenta flower color. Plant Molecular Biology 63: 125135.CrossRefGoogle ScholarPubMed
Takahashi, R, Matsumura, H, Oyoo, ME and Khan, NA (2008) Genetic and linkage analysis of purple-blue flower in soybean. Journal of Heredity 99: 593597.CrossRefGoogle Scholar
Takahashi, R, Dubouzet, JG, Matsumura, H, Yasuda, K and Iwashina, T (2010) A new allele of flower color gene W1 encoding flavonoid 3′5′-hydroxylase is responsible for light purple flowers in wild soybean Glycine soja . BMC Plant Biology 10: 155.CrossRefGoogle Scholar
Takahashi, R, Benitez, ER, Oyoo, ME, Khan, NA and Komatsu, S (2011) Nonsense mutation of an MYB transcription factor is associated with purple-blue flower color in soybean. Journal of Heredity 102: 458463.CrossRefGoogle Scholar
Tamura, K and Nei, M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512526.Google ScholarPubMed
Tamura, K, Peterson, D, Peterson, N, Stecher, G, Nei, M and Kumar, S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 27312739.CrossRefGoogle ScholarPubMed
Wang, KJ, Li, XH, Zhang, JJ, Chen, H, Zhang, ZL and Yu, GD (2010) Natural introgression from cultivated soybean (Glycine max) into wild soybean (Glycine soja) with the implications for origin of populations of semi-wild type and for biosafety of wild species in China. Genetic Resources and Crop Evolution 57: 747761.CrossRefGoogle Scholar
Wen, Z, Ding, Y and Zhao, T (2009) Genetic diversity and peculiarity of annual wild soybean (G. soja Sieb. et Zucc.) from various eco-regions in China. Theoretical and Applied Genetics 119: 371381.CrossRefGoogle Scholar
Xu, DH and Gai, JY (2003) Genetic diversity of wild and cultivated soybeans growing in China revealed by RAPD analysis. Plant Breeding 122: 503506.CrossRefGoogle Scholar
Xu, M, Brar, HK, Grosic, S, Palmer, RG and Bhattacharyya, MK (2010) Excision of an active CACTA-like transposable element DFR2 causes variegated flowers in soybean (Glycine max (L.) Merr.). Genetics 184: 5363.CrossRefGoogle Scholar
Yan, F, Di, S, Rojas Rodas, F, Rodriguez Torrico, T, Murai, Y, Iwashina, T, Anai, T and Takahashi, R (2014) Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2. BMC Plant Biology 14: 58.CrossRefGoogle ScholarPubMed
Zabala, G and Vodkin, LO (2005) The wp mutation of Glycine max carries a Gene-Fragment-Rich transposon of the CACTA superfamily. Plant Cell 17: 26192632.CrossRefGoogle ScholarPubMed
Zabala, G and Vodkin, LO (2007) A rearrangement resulting in small tandem repeats in the F3′5′H gene of white flower genotypes is associated with the soybean W1 locus. Plant Genome (A supplement to Crop Science) 47: S113S124.Google Scholar
Supplementary material: PDF

Park Supplementary Material

Supplementary Material

Download Park Supplementary Material(PDF)
PDF 581 KB
5
Cited by

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Diversity of the W1 gene encoding flavonoid 3′,5′-hydroxylase in white- and purple-flowered soybeans
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Diversity of the W1 gene encoding flavonoid 3′,5′-hydroxylase in white- and purple-flowered soybeans
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Diversity of the W1 gene encoding flavonoid 3′,5′-hydroxylase in white- and purple-flowered soybeans
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *