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Development and characterization of Triticum turgidum–Aegilops umbellulata amphidiploids

Published online by Cambridge University Press:  18 September 2018

Zhongping Song ,
Shoufen Dai ,
Yanni Jia ,
Li Zhao ,
Liangzhu Kang ,
Dengcai Liu ,
Yuming Wei ,
Youliang Zheng  and
Zehong Yan
Zhongping Song
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Shoufen Dai
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Yanni Jia
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Li Zhao
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Liangzhu Kang
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Dengcai Liu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Yuming Wei
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Youliang Zheng
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Zehong Yan*
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
*
*Corresponding author. E-mail: zhyan104@163.com
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Abstract

The U genome of Aegilops umbellulata is an important basic genome of genus Aegilops. Direct gene transfer from Ae. umbellulata into wheat is feasible but not easy. Triticum turgidum–Ae. umbellulata amphidiploids can act as bridges to circumvent obstacles involving direct gene transfer. Seven T. turgidum–Ae. umbellulata amphidiploids were produced via unreduced gametes for spontaneous doubling of chromosomes of triploid T. turgidum–Ae. umbellulata F1 hybrid plants. Seven pairs of U chromosomes of Ae. umbellulata were distinguished by fluorescence in situ hybridization (FISH) probes pSc119.2/(AAC)5 and pTa71. Polymorphic FISH signals were detected in three (1U, 6U and 7U) of seven U chromosomes of four Ae. umbellulata accessions. The chromosomes of the tetraploid wheat parents could be differentiated by probes pSc119.2 and pTa535, and identical FISH signals were observed among the three accessions. All the parental chromosomes of the amphidiploids could be precisely identified by probe combinations pSc119.2/pTa535 and pTa71/(AAC)5. The T. turgidum–Ae. umbellulata amphidiploids possess valuable traits for wheat improvement, such as strong tillering ability, stripe rust resistance and seed size-related traits. These materials can be used as media in gene transfers from Ae. umbellulata into wheat.

Keywords

amphidiploidsFISH (fluorescent in situ hybridization)T. turgidum ssp.U genomeunreduced gametes
Type
Research Article
Information
Plant Genetic Resources , Volume 17 , Issue 1 , February 2019 , pp. 24 - 32
Copyright
Copyright © NIAB 2018 

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Footnotes

†

Contributed equally to this work.

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