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Characterization of novel low-molecular-weight glutenin subunit genes from the diploid wild wheat relative Aegilops umbellulata

Published online by Cambridge University Press:  12 May 2022

Wenyang Wang
Affiliation:
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, China Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Wenjun Ji
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Lihua Feng
Affiliation:
College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China
Shunzong Ning
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Zhongwei Yuan
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Ming Hao
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Lianquan Zhang
Affiliation:
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, China Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Zehong Yan
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Bihua Wu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Dengcai Liu
Affiliation:
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, China Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Lin Huang*
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
*
Author for correspondence: Lin Huang, E-mail: lhuang@sicau.edu.cn

Abstract

Low molecular weight glutenin subunits (LWM-GSs) play a crucial role in determining wheat flour processing quality. In this work, 35 novel LMW-GS genes (32 active and three pseudogenes) from three Aegilops umbellulata (2n = 2x = 14, UU) accessions were amplified by allelic-specific PCR. We found that all LMW-GS genes had the same primary structure shared by other known LMW-GSs. Thirty-two active genes encode 31 typical LMW-m-type subunits. The MZ424050 possessed nine cysteine residues with an extra cysteine residue located in the last amino acid residue of the conserved C-terminal III, which could benefit the formation of larger glutenin polymers, and therefore may have positive effects on dough properties. We have found extensive variations which were mainly resulted from single-nucleotide polymorphisms (SNPs) and insertions and deletions (InDels) among the LMW-GS genes in Ae. umbellulata. Our results demonstrated that Ae. umbellulata is an important source of LMW-GS variants and the potential value of the novel LMW-GS alleles for wheat quality improvement.

Type
Research Article
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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Footnotes

*

Wenyang Wang and Wenjun Ji have contributed equally to this work.

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Characterization of novel low-molecular-weight glutenin subunit genes from the diploid wild wheat relative Aegilops umbellulata
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