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Assessing heat stress tolerance and genetic diversity among exotic and Indian wheat genotypes using simple sequence repeats and agro-physiological traits

Published online by Cambridge University Press:  23 November 2015

K. T. Ramya
Affiliation:
Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012, India
Neelu Jain
Affiliation:
Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012, India
Nikita Gandhi
Affiliation:
Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012, India
Ajay Arora
Affiliation:
Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110 012, India
P. K. Singh
Affiliation:
Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012, India
Anju M. Singh
Affiliation:
Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012, India
Gyanendra P. Singh
Affiliation:
Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012, India
K. V. Prabhu
Affiliation:
Directorate, Indian Agricultural Research Institute, New Delhi 110 012, India
Corresponding

Abstract

Genetic diversity and relationship of 92 bread wheat (Triticum aestivum L.) genotypes from India and exotic collections were examined using simple sequence repeat (SSR) markers and phenotypic traits to identify new sources of diversity that could accelerate the development of improved wheat varieties better suited to meet the challenges posed by heat stress in India. Genetic diversity assessed by using 82 SSR markers was compared with diversity evaluated using five physiological and six agronomic traits under the heat stress condition. A total of 248 alleles were detected, with a range of two to eight alleles per locus. The average polymorphic information content value was 0.37, with a range of 0.04 (cfd9) to 0.68 (wmc339). The heat susceptibility index was determined for grain yield per spike, and the genotypes were grouped into four categories. Two dendrograms that were constructed based on phenotypic and molecular analysis using UPGMA (unweighted pair group method with arithmetic mean) were found to be topologically different. Genotypes characterized as highly heat tolerant were distributed among all the SSR-based cluster groups. This implies that the genetic basis of heat stress tolerance in these genotypes is different, thereby enabling wheat breeders to combine these diverse sources of genetic variability to improve heat tolerance in their breeding programmes.

Type
Research Article
Copyright
Copyright © NIAB 2015 

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Footnotes

#

Present address: ICAR-Indian Institute of Oilseeds Research, Hyderabad 500030, India

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