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Assessing genetic diversity of potato genotypes using inter-PBS retrotransposon marker system

Published online by Cambridge University Press:  21 February 2017

Ufuk Demirel
Affiliation:
Department of Agricultural Genetic Engineering, Ömer Halisdemir University, 51240 Nigde, Turkey
İlknur Tındaş
Affiliation:
Department of Agricultural Genetic Engineering, Ömer Halisdemir University, 51240 Nigde, Turkey
Caner Yavuz
Affiliation:
Department of Agricultural Genetic Engineering, Ömer Halisdemir University, 51240 Nigde, Turkey
Faheem Shehzad Baloch
Affiliation:
Department of Field Crops, Abant İzzet Baysal University, Bolu, Turkey
Mehmet Emin Çalışkan
Affiliation:
Department of Agricultural Genetic Engineering, Ömer Halisdemir University, 51240 Nigde, Turkey
Corresponding
E-mail address:

Abstract

Having knowledge on genetic similarity and DNA profile of potato genotypes facilitates a breeder's decision for parent selection and provides accurate variety identification. Fingerprinting and identification of genetic similarity among 151 potato genotypes were achieved using an inter-primer-binding sites (iPBS) retrotransposon marker system. Our study is the first application of iPBS markers for fingerprinting and distinguishing large numbers of Solanum tuberosum genotypes. Initially, 16 potato genotypes were screened using 45 iPBS retrotransposon markers to identify polymorphisms. Seventeen of these primers were selected for fingerprinting the whole set of accessions due to strong, reproducible and polymorphic bands. The 17 iPBS primers produced 290 scorable bands of which 224 were polymorphic. The number of bands per primer ranged from 10 to 26 with an average of 17.1. The number of polymorphic bands per primer was between 6 and 21. The polymorphism percentage per primer ranged from 46.2 to 100.0% with an average of 77.2% per primer. The mean polymorphism information content (PIC) values of iPBS primers varied from 0.12 to 0.31 per primer. Genetic similarity based on Jaccard's coefficient of potato genotypes ranged from 0.61 to 0.93 with an average of 0.73. The data produced herein may be used for selection of appropriate parents and variety description in the future. The findings of the present study suggest that iPBS retrotransposons are powerful and easy DNA markers for fingerprinting the large samples of potato germplasm.

Type
Research Article
Copyright
Copyright © NIAB 2017 

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