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Vitamin supplementation increases the virulence of Entamoeba histolytica grown axenically

Published online by Cambridge University Press:  23 January 2020

Javier Vargas-Villareal ,
Gerardo Lozano-Garza ,
Salvador Luis Said-Fernández ,
Francisco González-Salazar ,
María Guadalupe Moreno-Treviño ,
Ofelia Monsiváis-Diaz ,
Alan Giresse Lozano-Alanís  and
Francisco Javier Guzmán-de la Garza Open the ORCID record for Francisco Javier Guzmán-de la Garza [Opens in a new window]
Javier Vargas-Villareal
Affiliation:
Laboratorio de Biología Celular/Centro de Investigación Biomédica del Noreste. Delegación Nuevo León, Instituto Mexicano del Seguro Social, Calle 2 de Abril #501, Col. Independencia, CP 64720, Monterrey, Nuevo León, México Departamento de Ciencias Básicas, Vicerrectoría de Ciencias de la Salud, Universidad de Monterrey, Monterrey, Nuevo León, México
Gerardo Lozano-Garza*
Affiliation:
Laboratorio de Biología Celular/Centro de Investigación Biomédica del Noreste. Delegación Nuevo León, Instituto Mexicano del Seguro Social, Calle 2 de Abril #501, Col. Independencia, CP 64720, Monterrey, Nuevo León, México
Salvador Luis Said-Fernández
Affiliation:
Departamento de Bioquímica y Medicina Molecular, Universidad Autónoma de Nuevo León; Facultad de Medicina, Calle Gonzalitos #235 norte, Col. Mitras centro, CP 64460, MonterreyNuevo León, México
Francisco González-Salazar
Affiliation:
Laboratorio de Biología Celular/Centro de Investigación Biomédica del Noreste. Delegación Nuevo León, Instituto Mexicano del Seguro Social, Calle 2 de Abril #501, Col. Independencia, CP 64720, Monterrey, Nuevo León, México Departamento de Ciencias Básicas, Vicerrectoría de Ciencias de la Salud, Universidad de Monterrey, Monterrey, Nuevo León, México
María Guadalupe Moreno-Treviño
Affiliation:
Departamento de Ciencias Básicas, Vicerrectoría de Ciencias de la Salud, Universidad de Monterrey, Monterrey, Nuevo León, México
Ofelia Monsiváis-Diaz
Affiliation:
Laboratorio de Biología Celular/Centro de Investigación Biomédica del Noreste. Delegación Nuevo León, Instituto Mexicano del Seguro Social, Calle 2 de Abril #501, Col. Independencia, CP 64720, Monterrey, Nuevo León, México
Alan Giresse Lozano-Alanís
Affiliation:
Departamento de Fisiología, Universidad Autónoma de Nuevo León; Facultad de Medicina, Calle Gonzalitos #235 norte, Col. Mitras centro, CP 64460, MonterreyNuevo León, México
Francisco Javier Guzmán-de la Garza*
Affiliation:
Laboratorio de Biología Celular/Centro de Investigación Biomédica del Noreste. Delegación Nuevo León, Instituto Mexicano del Seguro Social, Calle 2 de Abril #501, Col. Independencia, CP 64720, Monterrey, Nuevo León, México Departamento de Fisiología, Universidad Autónoma de Nuevo León; Facultad de Medicina, Calle Gonzalitos #235 norte, Col. Mitras centro, CP 64460, MonterreyNuevo León, México
*
Author for correspondence: Francisco Javier Guzmán-de la Garza, E-mail: fcojguzman@hotmail.com
Author for correspondence: Francisco Javier Guzmán-de la Garza, E-mail: fcojguzman@hotmail.com
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Abstract

As a consequence of axenic growth and the elimination of accompanying bacterial flora, Entamoeba histolytica virulence decreases rapidly, and pathogenicity is lost. This paper evaluated the impact of vitamin supplementation on the pathogenicity of E. histolytica. Growth of E. histolytica trophozoites, cultured axenically in PEHPS (a Spanish acronym for the main ingredients – casein peptone, liver, pancreas extract and bovine serum) medium, with or without vitamins, exhibited a similar growth rate. However, the vitamin-enriched PEHPS preparations expressed 2.65 times more haemolytic activity (at 60 min: 98 vs 48%, P < 0.05), 2.5 times more phospholipase A2 activity at 150 min of incubation and generated more hepatic abscesses (88 vs 60%, P = 0.05) than the preparations without vitamins. The haemolytic and phospholipase A2 activity for the PEHPS − V preparations were restored following vitamin supplementation with A and D. These data highlight, for the first time, that vitamins and specifically vitamin A and D were essential for the recovery of amoebic virulence, lost through axenic growth.

Keywords

Entamoeba histolytica growthPEHPS culture mediavitamin Avitamin Dvitamins increase virulence
Type
Research Article
Information
Parasitology , Volume 147 , Issue 4 , April 2020 , pp. 501 - 505
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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