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Trypanosoma cruzi isolates from Chile are heterogeneous and composed of mixed populations when characterized by schizodeme and Southern analyses

Published online by Cambridge University Press:  01 March 2004

J. P. TORRES ,
S. ORTIZ ,
S. MUÑOZ  and
A. SOLARI
J. P. TORRES
Affiliation:
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Casilla 70086, Santiago 7, Chile
S. ORTIZ
Affiliation:
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Casilla 70086, Santiago 7, Chile
S. MUÑOZ
Affiliation:
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Casilla 70086, Santiago 7, Chile
A. SOLARI
Affiliation:
Program of Cellular and Molecular Biology, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Casilla 70086, Santiago 7, Chile
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Abstract

In total, 61 Chilean isolates of Trypanosoma cruzi, were analysed using schizodeme and Southern analysis, using as probes the highly variable regions of minicircles from cloned parasites. Isolates were collected and amplified from domestic and wild triatomines, and from infected subjects in all the endemic areas of Chile. Three major parasite genotypes could be detected in the domestic transmission cycle, whilst 1 major T. cruzi genotype is circulating in the wild transmission cycle. Schizodeme analysis suggested that T. cruzi isolates are mixed populations, whereas the Southern analyses detected only 3 mixed isolates using 4 selected minicircle segments as probes.

Keywords

Southern analysisschizodemesmixed populationsTrypanosoma cruzi
Type
Research Article
Information
Parasitology , Volume 128 , Issue 2 , February 2004 , pp. 161 - 168
Copyright
2004 Cambridge University Press

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