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Structural modelling studies and immunoprophylactic potential of Brugia malayi DEAD Box RNA helicase

Published online by Cambridge University Press:  07 June 2013

MEGHNA SINGH ,
NIDHI SHRIVASTAVA ,
UZMA SAQIB ,
MOHAMMAD IMRAN SIDDIQI  and
SHAILJA MISRA-BHATTACHARYA
MEGHNA SINGH
Affiliation:
Division of Parasitology, CSIR-Central Drug Research Institute, BS10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow 226021, Uttar Pradesh, India
NIDHI SHRIVASTAVA
Affiliation:
Division of Parasitology, CSIR-Central Drug Research Institute, BS10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow 226021, Uttar Pradesh, India
UZMA SAQIB
Affiliation:
Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, BS10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow 226021, Uttar Pradesh, India
MOHAMMAD IMRAN SIDDIQI
Affiliation:
Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, BS10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow 226021, Uttar Pradesh, India
SHAILJA MISRA-BHATTACHARYA*
Affiliation:
Division of Parasitology, CSIR-Central Drug Research Institute, BS10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow 226021, Uttar Pradesh, India
*
*Corresponding author: Division of Parasitology, Central Drug Research Institute, Lucknow-226021, UP, India. Tel: +91 522 2612411 18. PABX 4221/4224. Fax: +91 522 2623405/2623938/2629504. E-mail: shailja_cdri@rediffmail.com, shailjacdri@yahoo.com
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Summary

DEAD Box RNA helicases are essential enzymes that are involved in RNA metabolic processes such as transcription, pre-mRNA splicing, translation initiation and RNA decay. We have previously over-expressed and biochemically characterized an immunodominant cDNA clone encoding DEAD box RNA helicase (BmL3-Helicase) isolated by immunoscreening of the larval stage cDNA library of Brugia malayi. In the current study, the 3D structure was determined and the immunoprophylactic efficacy of BmL3-Helicase was investigated by immunizing Mastomys coucha with the recombinant protein and subsequently challenging with B. malayi infective larvae. The immunization had an adverse outcome on the establishment of challenged larvae resulting in a 67·4% reduction in adult parasite recovery, a 86·7% decrease in the microfilarial density and profound sterility of the recovered female worms. The immune response thus generated was investigated by measuring the levels of specific antibodies including IgG subclasses, reactive oxygen species and cytokines.

Keywords

BmL3-Helicasehomology modellingimmunoprophylaxis
Type
Research Article
Information
Parasitology , Volume 140 , Issue 8 , July 2013 , pp. 1016 - 1025
Copyright
Copyright © Cambridge University Press 2013 

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