Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-25T06:03:29.277Z Has data issue: false hasContentIssue false
  • English
  • Français

Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis)

Published online by Cambridge University Press:  11 August 2014

J. P. DUBEY ,
M. HILALI ,
E. VAN WILPE ,
S. K. VERMA ,
R. CALERO-BERNAL  and
A. ABDEL-WAHAB
J. P. DUBEY*
Affiliation:
U. S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA
M. HILALI
Affiliation:
Parasitology Department, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt
E. VAN WILPE
Affiliation:
Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa
S. K. VERMA
Affiliation:
U. S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA
R. CALERO-BERNAL
Affiliation:
U. S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA
A. ABDEL-WAHAB
Affiliation:
Parasitology Department, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt
*
*Corresponding author: USDA, ARS, APDL, BARC-East, Building 1001, Beltsville, MD 20705, USA. E-mail: jitender.dubey@ars.usda.gov
Get access Rights & Permissions [Opens in a new window]

Summary

Four valid species of Sarcocystis have been reported from the water buffalo (Bubalus bubalis): Sarcocystis fusiformis, Sarcocystis buffalonis, Sarcocystis levinei and Sarcocystis dubeyi. Here, we redescribe structure of S. fusiformis sarcocysts by scanning and transmission electron microscopy (SEM, TEM). Twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in Egypt were examined by light microscopy, SEM and TEM. The sarcocyst wall was up to 9 μm thick, depending on the section and the technique. In 5 μm paraffin-embedded sections, the sarcocyst wall was indistinct, 2–5 μm thick and appeared smooth. In 1 μm plastic-embedded sections stained with toluidine blue, the sarcocyst wall was 2·5–5·2 μm thick and had branched villar protrusions (vp)-like branches of a dead tree. By SEM, the sarcocyst wall had a mesh-like structure with irregularly shaped vp that were folded over the sarcocyst wall. On each vp there were uniform papillomatous structures that were 100 nm wide. By TEM, vp were up to 6 μm long and contained filamentous tubular structures, most of which were parallel to the long axis of the projections; granules were absent from these tubules. By TEM, bradyzoites within the same cyst varied from 11·2 to 16·8 μm in length. By TEM, bradyzoites had a very long (10 μm) convoluted mitochondrion, up to 12 dense granules, but only 2 rhoptries. This redescription should help to differentiate the sarcocysts of S. fusiformis from similar sarcocysts in domestic and wild ruminants.

Keywords

Sarcocystis fusiformissarcocystwater buffalo (Bubalus bubalis)electron microscopyultrastructure
Type
Research Article
Information
Parasitology , Volume 142 , Issue 2 , February 2015 , pp. 385 - 394
Check for updates
Creative Commons
This is a work of the U.S. Government and is not subject to copyright protection in the United States.
Copyright
Copyright © Cambridge University Press 2014

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Chen, X., Zuo, Y., Rosenthal, B. M., He, Y., Cui, L. and Yang, Z. (2011). Sarcocystis sinensis is an ultrastructurally distinct parasite of water buffalo that can cause foodborne illness but cannot complete its life-cycle in human beings. Veterinary Parasitology 178, 35–39.CrossRefGoogle ScholarPubMed
Dissanaike, A. S. and Kan, S. P. (1978). Studies on Sarcocystis in Malaysia. I. Sarcocystis levinei n. sp. from the water buffalo Bubalus bubalis . Zeitschrift für Parasitenkunde (now Parasitology Research) 55, 127–138.CrossRefGoogle Scholar
Dubey, J. P., Speer, C. A. and Fayer, R. (1989 a). Sarcocystosis of Animals and Man, pp. 1–215. CRC Press, Boca Raton, FL, USA.Google Scholar
Dubey, J. P., Speer, C. A. and Shah, H. L. (1989 b). Ultrastructure of sarcocysts from water buffalo in India. Veterinary Parasitology 34, 149–152.CrossRefGoogle ScholarPubMed
Dubey, J. P., Fayer, R., Rosenthal, B. M., Calero-Bernal, R. and Uggla, A. (2014). Identity of Sarcocystis species of the water buffalo (Bubalus bubalis) and cattle and the suppression of Sarcocystis sinensis as a nomen nudum . Veterinary Parasitology (in press). doi: 10.1016/j.vetpar.2014.06.020 CrossRefGoogle ScholarPubMed
Ghaffar, F. A., Hilali, M. and Scholtyseck, E. (1978). Ultrastructural study of Sarcocystis fusiformis (Railliet,1897) infecting the Indian water buffalo (Bubalus bubalis) of Egypt. Tropenmedizin und Parasitologie 29, 289–294.Google ScholarPubMed
Gjerde, B. (2013). Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. International Journal for Parasitology 43, 579–591.CrossRefGoogle ScholarPubMed
Heydorn, A. O., Gestrich, R., Mehlhorn, H. and Rommel, M. (1975). Proposal for a new nomenclature of the Sarcosporidia. Zeitschrift für Parasitenkunde (now Parasitology Research) 48, 73–82.CrossRefGoogle ScholarPubMed
Huong, L. T. T. (1999 a). Sarcocystis infections of the water buffalo in Vietnam. Doctoral thesis. Swedish University of Agricultural Sciences, Uppsala, Sweden, pp. 1–53.Google Scholar
Huong, L. T. T. (1999 b). Prevalence of Sarcocystis spp. in water buffaloes in Vietnam. Veterinary Parasitology 86, 33–39.CrossRefGoogle ScholarPubMed
Huong, L. T. T. and Uggla, A. (1999). Sarcocystis dubeyi n. sp. (Protozoa: Sarcocystidae) in the water buffalo (Bubalus bubalis). Journal of Parasitology 85, 102–104.CrossRefGoogle Scholar
Huong, L. T. T., Dubey, J. P., Nikkilä, T. and Uggla, A. (1997 a). Sarcocystis buffalonis n. sp. (Protozoa: Sarcocystidae) from the water buffalo (Bubalus bubalis) in Vietnam. Journal of Parasitology 83, 471–474.CrossRefGoogle Scholar
Huong, L. T. T., Dubey, J. P. and Uggla, A. (1997 b). Redescription of Sarcocystis levinei Dissanaike and Kan, 1978 (Protozoa: Sarcocystidae) of the water buffalo (Bubalus bubalis). Journal of Parasitology 83, 1148–1151.CrossRefGoogle ScholarPubMed
Jehle, C., Dinkel, A., Sander, A., Morent, M., Romig, T., Luc, P. V., De, T. V., Thai, V. V. and Mackenstedt, U. (2009). Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in northern Vietnam. Veterinary Parasitology 166, 314–320.CrossRefGoogle Scholar
Kan, S. P. and Dissanaike, A. S. (1978). Studies on Sarcocystis in Malaysia II. Comparative ultrastructure of the cyst wall and zoites of Sarcocystis levinei and Sarcocystis fusiformis from the water buffalo, Bubalus bubalis . Zeitschrift für Parasitenkunde (now Parasitology Research) 57, 107–116.CrossRefGoogle Scholar
Levine, N. D. (1977). Nomenclature of Sarcocystis in the ox and sheep and of fecal coccidia of the dog and cat. Journal of Parasitology 63, 36–51.CrossRefGoogle ScholarPubMed
Moré, G., Pantchev, A., Skuballa, J., Langenmayer, M. C., Maksimov, P., Conraths, F. J., Venturini, M. C. and Schares, G. (2014). Sarcocystis sinensis is the most prevalent thick-walled Sarcocystis in beef for consumers in Germany. Parasitology Research 113, 2223–2230.CrossRefGoogle ScholarPubMed
Parairo, J. R., Manuel, M. F. and Icatlo, F. C. (1988). Ultrastructural studies of the cyst wall of Sarcocystis spp. In Philippine carabaos. Philippine Journal of Veterinary and Animal Science 14, 40–54.Google Scholar
Wang, M., Liu, H. H., Lin, Q. W., Xiao, B. N., Zhang, C. G. and Gong, Z. F. (1989). A comparative ultrastructural study on the two types of sarcocysts in Chinese buffaloes. Acta Veterinaria et Zootechnica Sinica 20, 356–362.Google Scholar
Zaman, V. and Colley, F. C. (1972). Fine structure of Sarcocystis fusiformis from the Indian water buffalo (Bubalus bubalis) in Singapore. Southeast Asian Journal of Tropical Medicine and Public Health 3, 489–495.Google Scholar
Zaman, V., Robertson, T. A. and Papadimitriou, J. M. (1980). Scanning electron microscopy of Sarcocystis fusiformis from the water buffalo (Bubalus bubalis). Southeast Asian Journal of Tropical Medicine and Public Health 11, 205–211.Google ScholarPubMed