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Malaria toxins: effects on murine spleen and bone marrow cell proliferation and cytokine production in vitro

Published online by Cambridge University Press:  01 November 1997

G. BORDMANN ,
N. FAVRE  and
W. RUDIN
G. BORDMANN
Affiliation:
Swiss Tropical Institute, PO Box, 4002 Basel, Switzerland
N. FAVRE
Affiliation:
Swiss Tropical Institute, PO Box, 4002 Basel, Switzerland
W. RUDIN
Affiliation:
Swiss Tropical Institute, PO Box, 4002 Basel, Switzerland
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Abstract

The ability of deproteinated malaria exoantigens from Plasmodium falciparum (Pf-MT) and P. berghei ANKA (PbA-MT) to activate murine haematopoietic cells was analysed in vitro. Malaria toxins (MT) of both plasmodium species induced cell proliferation and the production of IFN-γ in overnight and long-term (5 days) spleen and bone marrow cultures and a reduction of the number of TNF-α spot forming cells (SFC). When added to cells of malaria-experienced animals, MT decreased the number of IL-4 SPC and increased the number of IL-5 SPC. However, the same proliferative and IFN-γ induction properties as in naive cells were observed. Simultaneous addition of IL-2 and PbA-MT to spleen cells inhibited the proliferation but increased the IFN-γ production usually induced by IL-2. Flow cytometric analysis revealed that the addition of MT triggered an expansion of CD3+ and GR1+ cell populations. Our results suggest that malaria toxins of different species can induce an immediate and strong proliferation and a TH1-type cytokine release by murine cells, independently of previous in vivo priming.

Keywords

malaria toxinsPlasmodium falciparumPlasmodium berghei ANKAmurine haematopoiesisimmunity
Type
Research Article
Information
Parasitology , Volume 115 , Issue 5 , November 1997 , pp. 475 - 483
Copyright
1997 Cambridge University Press

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