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Identification of Trichinella spiralis early antigens at the pre-adult and adult stages

Published online by Cambridge University Press:  19 November 2010

ALEKSANDAR ZOCEVIC ,
PAULINE MACE ,
ISABELLE VALLEE ,
RADU BLAGA ,
MINGYUAN LIU ,
SANDRINE A. LACOUR  and
PASCAL BOIREAU
ALEKSANDAR ZOCEVIC*
Affiliation:
AFSSA, ENVA, UPEC, LERPAZ, JRU BIPAR, 23 avenue du Général de Gaulle, 94700 Maisons-Alfort, France
PAULINE MACE
Affiliation:
AFSSA, ENVA, UPEC, LERPAZ, JRU BIPAR, 23 avenue du Général de Gaulle, 94700 Maisons-Alfort, France
ISABELLE VALLEE
Affiliation:
AFSSA, ENVA, UPEC, LERPAZ, JRU BIPAR, 23 avenue du Général de Gaulle, 94700 Maisons-Alfort, France
RADU BLAGA
Affiliation:
AFSSA, ENVA, UPEC, LERPAZ, JRU BIPAR, 23 avenue du Général de Gaulle, 94700 Maisons-Alfort, France
MINGYUAN LIU
Affiliation:
Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, 130062 Changchun, P.R.China
SANDRINE A. LACOUR
Affiliation:
AFSSA, ENVA, UPEC, LERPAZ, JRU BIPAR, 23 avenue du Général de Gaulle, 94700 Maisons-Alfort, France
PASCAL BOIREAU
Affiliation:
AFSSA, ENVA, UPEC, LERPAZ, JRU BIPAR, 23 avenue du Général de Gaulle, 94700 Maisons-Alfort, France
*
*Corresponding author: AFSSA, ENVA, UPEC, LERPAZ, JRU BIPAR, 23 avenue du Général de Gaulle, 94700 Maisons-Alfort, France. E-mail: a.zocevic@gmail.com
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Summary

Three expression cDNA libraries from Trichinella spiralis worms 14 h, 20 h and 48 h post-infection (p.i.) were screened with serum from pigs experimentally infected with 20 000 T. spiralis muscle larvae. Twenty-nine positive clones were isolated from the 14 h p.i. cDNA library, corresponding to 8 different genes. A putative excretory-secretory protein similar to that of T. pseudospiralis was identified. Three clones corresponded to a T. spiralis serine proteinase inhibitor known to be involved in diverse functions such as blood coagulation and modulation of inflammation. Screening of the 20 h p.i. cDNA library selected 167 positive clones representing 12 different sequences. The clone with the highest redundancy encoded a small polypeptide having no sequence identity with any known proteins from Trichinella or other organisms. Fourteen clones displayed sequence identity with the heat shock protein (HSP) 70. HSPs are produced as an adaptive response of the parasite to the hostile environment encountered in the host intestine but their mechanism of action is not yet well defined. From the 48 h p.i. T. spiralis cDNA library, 91 positive clones were identified representing 7 distinct sequences. Most of the positive clones showed high similarity with a member of a putative T. spiralis serine protease family. This result is consistent with a possible major role for serine proteases during invasive stages of Trichinella infection and host-parasite interactions.

Keywords

Trichinella spiraliscDNA libraryscreeningantigens
Type
Research Article
Information
Parasitology , Volume 138 , Issue 4 , April 2011 , pp. 463 - 471
Copyright
Copyright © Cambridge University Press 2010

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