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The excystation of Entamoeba histolytica without bacteria in microcultures

Published online by Cambridge University Press:  07 April 2009

Charles W. Rees ,
Lucy V. Reardon  and
Ida Louise Bartgis
Charles W. Rees
Affiliation:
From the Laboratory of Tropical Diseases, National Institutes of Health, Bethesda, Maryland
Lucy V. Reardon
Affiliation:
From the Laboratory of Tropical Diseases, National Institutes of Health, Bethesda, Maryland
Ida Louise Bartgis
Affiliation:
From the Laboratory of Tropical Diseases, National Institutes of Health, Bethesda, Maryland
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Extract

1. Formulae developed by Anfinsen et al. (1946) for a medium used in the cultivation of Plasmodium knowlesi were used for media in which excystation of Entamoeba histolytica without bacteria was investigated.

2. The following media were used: (i) an inorganic fluid containing carbon dioxide and chlorides, phosphates, and bicarbonates, of sodium, potassium, magnesium, and calcium; (ii) an inorganic fluid of chlorides and phosphates of the above metals without bicarbonates; (iii) the same respective fluids plus glucose, and (iv) the same plus enrichments with B vitamins, cocarboxylase, vitamin C, purines, pyrimidines, folic acid, glucosamine, cholesterol, and amino-acids. The oxygen content of all media was lowered either by cysteine or glutathione.

3. Some excystation occurred in all media, the percentages were lowest in the inorganic fluid without bicarbonates, highest in fluid with bicarbonates plus all of the listed organic compounds, and intermediate in the inorganic bicarbonate fluid plus glucose. Amino-acids were not required for good percentages of excystation.

4. Excystation occurred when cysts were isolated in medium with Trypanosoma cruzi.

5. The data show that organic compounds in the medium are necessary for high percentages of excystation.

Type
Research Article
Information
Parasitology , Volume 40 , Issue 3-4 , October 1950 , pp. 338 - 342
Copyright
Copyright © Cambridge University Press 1950

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