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The effect of iron on Trichomonas vaginalis TvCP2: a cysteine proteinase found in vaginal secretions of trichomoniasis patients

Published online by Cambridge University Press:  16 March 2020

Luis Alberto Rivera-Rivas ,
Sebastián Lorenzo-Benito ,
Diana Belén Sánchez-Rodríguez ,
Jesús FT Miranda-Ozuna ,
Esly Alejandra Euceda-Padilla ,
Jaime Ortega-López ,
Bibiana Chávez-Munguía ,
Anel Lagunes-Guillén ,
Beatriz Velázquez-Valassi  and
Lidia Jasso-Villazul
...Show all authors
Luis Alberto Rivera-Rivas
Affiliation:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), CP 07360, Mexico City, Mexico
Sebastián Lorenzo-Benito
Affiliation:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), CP 07360, Mexico City, Mexico
Diana Belén Sánchez-Rodríguez
Affiliation:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), CP 07360, Mexico City, Mexico
Jesús FT Miranda-Ozuna
Affiliation:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), CP 07360, Mexico City, Mexico
Esly Alejandra Euceda-Padilla
Affiliation:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), CP 07360, Mexico City, Mexico
Jaime Ortega-López
Affiliation:
Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN, CP 07360, Mexico City, Mexico
Bibiana Chávez-Munguía
Affiliation:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), CP 07360, Mexico City, Mexico
Anel Lagunes-Guillén
Affiliation:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), CP 07360, Mexico City, Mexico
Beatriz Velázquez-Valassi
Affiliation:
Departamento de Vigilancia Epidemiológica del Hospital General de México ‘Eduardo Liceaga’, CP 06720, Mexico City, Mexico
Lidia Jasso-Villazul
Affiliation:
Unidad de Medicina Preventiva del Hospital General de México ‘Eduardo Liceaga’, CP 06720, Mexico City, Mexico
Rossana Arroyo*
Affiliation:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), CP 07360, Mexico City, Mexico
*
Author for correspondence: Rossana Arroyo, E-mail: rarroyo@cinvestav.mx
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Abstract

Trichomonas vaginalis (Tv) induces host cell damage through cysteine proteinases (CPs) modulated by iron. An immunoproteomic analysis showed that trichomoniasis patient sera recognize various CPs, also some of them are present in vaginal washes (VWs). Thus, the goal of this work was to determine whether TvCP2 is expressed during infection and to assess the effect of iron on TvCP2 expression, localization and contribution to in vitro cellular damage. Western-blotting (WB) assays using TvCP2r and vaginitis patient serum samples showed that 6/9 Tv (+) but none of the Tv (−) patient sera recognized TvCP2r. WB using an anti-TvCP2r antibody and VWs from the same patients showed that in all of the Tv (+) but none of the Tv (−) VWs, the anti-TvCP2r antibody detected a 27 kDa protein band that corresponded to the mature TvCP2, which was confirmed by mass spectrometry analysis. Iron decreased the amount of TvCP2 mRNA and the protein localized on the parasite surface and cytoplasmic vesicles concomitant with the cytotoxic effect of TvCP2 on HeLa cells. Parasites pretreated with the anti-TvCP2r antibody also showed reduced levels of cytotoxicity and apoptosis induction in HeLa cell monolayers. In conclusion, these results show that TvCP2 is expressed during trichomonal infection and plays an important role in the in vitro HeLa cell cytotoxic damage under iron-restricted conditions.

Keywords

Cathepsin L-like cysteine proteinasescellular apoptosis inductioncytotoxicityimmunogenic proteinaseirontrichocystatin-3 (TC-3)Trichomonas vaginalisTvCP2vaginal secretions
Type
Research Article
Information
Parasitology , Volume 147 , Issue 7 , June 2020 , pp. 760 - 774
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Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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